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虹膜素通过促进由HIF-1α通路介导的视网膜微质M1到M2表型极化来缓解自身免疫性尿道炎

Meiqi Wang¹, Xue Huang¹, Jia Shu²

¹Department of Ophthalmology, Joint Research Laboratory for Ocular Immunology and Retinal Injury Repair, The First Affiliated Hospital of Zhengzhou University, Henan International, Henan Province Eye Hospital, Zhengzhou, P.R. China.

|September 29, 2024

概括

运动激素虹膜素在脑膜炎患者中降低. 补充虹膜素通过通过HIF-1α通路调节免疫细胞和微质细胞来减少炎症,为卵膜炎提供了潜在的治疗点.

科学领域:

眼科医生眼科眼科
免疫学免疫学免疫学
内分泌学在内分泌学.

背景情况:

素,源自Fndc5,是一种与运动相关的激素.
尿道炎是一组具有复杂病原性的炎症性眼病.
虹膜素在眼部炎症,特别是脑膜炎中的作用仍然在很大程度上未被探索.

研究的目的:

为了研究膜炎患者的水性幽默中的虹膜素水平.
阐明伊丽素在实验性自身免疫性脑膜炎 (EAU) 病变发生过程中的作用.
探索虹素在脑膜炎中的作用的潜在分子机制.

主要方法:

从Vogt-Koyanagi-Harada (VKH) 和Behcet脑膜炎 (BU) 患者的水性幽默中测量素水平.
在阿联的小鼠模型中给予外源性虹膜素.
评估EAU小鼠的临床和病理评分.
流细胞计分析T辅助细胞种群 (Th1,Th17).
在视网膜中分析微质极化 (M1/M2).
RNA测序以识别Fndc5淘汰EAU小鼠中的丰富途径.
在Fndc5淘汰赛EAU小鼠中使用HIF-1α通路抑制剂进行治疗.

主要成果:

在VKH和BU患者的水性幽默中,虹膜素水平显著降低.
在BU患者中,降低的虹膜素水平与TNF-α负相关.
外源性虹膜素补充剂改善了EAU的临床和病理症状.
虹膜素抑制了中的Th1和Th17细胞比例,并减轻了M1微质极化,同时促进了视网膜中的M2极化.
在Fndc5淘汰赛EAU小鼠中,炎症恶化和微质激活增加.
在Fndc5淘汰EAU小鼠中,HIF-1α通路被丰富,其抑制通过减少M1微质极化来挽救EAU严重性.

结论:

皮膜炎患者的水性幽默中虹膜素水平降低,这表明它起着保护作用.
虹膜素通过抑制促炎性Th1/Th17细胞和M1微质细胞,在实验性脑膜炎中发挥治疗作用.
素的保护机制涉及到视网膜中HIF-1α通路的调节.

关键词:

在HIF-1α通路上.虹膜是一种虹膜.这是一个令人惊的结果.微质细胞中的微质细胞

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