从Serratia marcescens提取和净化的Prodigiosin的抗癌和免疫调节活动
Sahira N Muslim1, Alaa Naseer Mohammed Ali1, Entkhab M A Alanisi1
1Department of Biology, College of Science, Mustansiriyah University, Baghdad, Iraq.
Asian Pacific journal of cancer prevention : APJCP
|September 29, 2024
概括
这项研究从Serratia marcescens中提取了prodigiosin,发现它有效地向癌细胞,同时增强免疫反应. 这种天然化合物既具有抗癌作用,又具有免疫调节作用.
科学领域:
- 微生物学 微生物学
- 生物化学 生化学
- 免疫学 免疫学 免疫学
背景情况:
- 产素是一种来自Serratia marcescens的天然化合物,具有多样化的生物活性.
- 之前的研究强调了它的潜力,需要对其治疗应用进行进一步的研究.
研究的目的:
- 为了提取和净化来自Serratia marcescens的奇异蛋白.
- 为了评估prodigiosin的抗癌和免疫调节作用.
主要方法:
- 使用各种溶剂提取Prodigiosin,其中甲醇产生最高度.
- 细胞毒性评价针对人类狂宫肌肉瘤,大鼠胚胎纤维细胞和MDA-MB-231细胞系.
- 在小鼠体内免疫调节分析,测量中性粒细胞数量和细胞因子水平 (IL-4,IL-10).
主要成果:
- 甲醇提取产生了65-230毫克/升的奇异.
- 普罗迪吉奥辛对癌细胞 (MDA上皮细胞) 具有选择性细胞毒性,但对正常细胞则没有.
- 显著增加IL-4和IL-10水平和改变的中性粒细胞数量被观察到 prodigiosin 治疗的小鼠.
结论:
- 谢拉蒂亚马尔塞森斯奇观素表现出强大的抗癌性质.
- 产素通过增加IL-4和IL-10血清水平,起到免疫调节剂的作用.
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