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在降雨期间,抗生素耐药性基因在土壤中的垂直迁移和浸出行为:长期受精的影响

Yu Zhang¹, Yuting Zhang², Jun Xie³

¹College of Resources and Environment, Southwest University, Chongqing, 400716, PR China; School of Agriculture and Biotechnology, Shenzhen Campus of SunYat-sen University, Shenzhen, 518107, PR China.

|September 29, 2024

概括

长期的肥施肥会增加抗生素耐药性基因 (ARG) 的迁移和土壤中的浸,对地下水构成风险. 酸性土壤在液中显示出最高的ARG负载.

科学领域:

环境微生物学环境微生物学
土壤科学土壤科学
遗传学是一个遗传学.

背景情况:

抗生素耐药性基因 (ARG) 构成了严重的环境和公共卫生威胁.
了解土壤中的ARG命运,特别是垂直迁移和浸,对于风险评估至关重要.
长期受精对ARG流动性的影响尚不清楚.

研究的目的:

在长期施肥下,研究ARG在不同类型的土壤中的垂直迁移和浸出.
量化肥对土壤水中的ARG负载的影响.
确定影响ARG运输的关键土壤特性和微生物宿主.

主要方法:

使用高通量定量PCR,在一年内对土壤样式 (0-60厘米) 和浸出物中的ARG进行监测.
从13年的田间施肥实验中分析了三种土壤类型 (酸性,中性,性).
使用SourceTracker和PiecewiseSEM分析来识别ARG源,主机和影响因素.

主要成果:

长期的肥肥料显著增加了特定的ARG (氨基糖化物,β-乳,多药物) 的垂直迁移和浸出.
排泄物中的年度累积ARG负载在受肥的土壤中较高,而酸性土壤的负载最大.
堆肥土壤是浸出物中ARG的主要来源,特定的细菌被确定为主导宿主.

结论:

长期的肥料施肥提高了土壤中的ARG流动性,增加了地下水污染的风险.
像pH值,有机碳和沙子含量等土壤属性介导ARG迁移.
需要管理策略来缓解ARG从受精土壤中的出.

关键词:

抗生素耐药性基因是抗生素耐药性的基因.溶解液是一种溶解液.肥受精肥受精肥受精肥受精土壤特征土地特征土壤特征垂直迁移是指垂直迁移的过程.

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