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使用富里埃变换红外光谱法 (FTIR) 量化分析Lycium barbarum多糖体 (LBPs) 的方法:从理论计算到实验应用

Zhuan Nan¹, Lulu Chen¹, Guangxia Li¹

¹School of Chemistry and Chemical Engineering, North Minzu University, Yinchuan 750021, China.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy

|September 29, 2024

概括

一种新的里埃变换红外光谱法 (FTIR) 方法量化了Lycium barbarum中的Lycium barbarum多糖体 (LBPs). 这种快速测定为分析植物多糖的传统方法提供了替代方案.

科学领域:

分析化学分析化学
频谱学是一种光谱学.
植物化学植物化学

背景情况:

草甘多糖 (LBPs) 是Lycium barbarum (LB) 中的主要生物活性化合物.
由于复杂的结构和缺乏参考标准,量化LBP具有挑战性.
现有的方法可能不足以准确或快速进行常规分析.

研究的目的:

建立一种使用富里埃变换红外光谱法 (FTIR) 在Lycium barbarum中LBPs的定量分析方法.
为了验证开发的FTIR方法的准确性,精度和可靠性.
为了将结果与传统方法比较,如-硫酸测定.

主要方法:

利用FTIR光谱学进行LBP的定量分析.
根据理论计算,选择了一个特征的吸收带 (921 cm-1).
采用德克斯作为分子量认证参考材料 (CRM) 和KSCN作为内部标准.
进行的方法验证,包括回收和空白回收试验.
确定的检测极限 (LOD) 和量化极限 (LOQ).

主要成果:

该FTIR方法表现出良好的精度 (RSD=1.10%) 和准确性 (恢复率:96.61%-105.45%).
对CRMD的LOD和LOQ分别为0.10毫克和0.32毫克.
通过FTIR确定的LBP含量低于在LB样本和提取物中通过硫酸测定得到的含量.
该方法表现出对准备变化和环境因素的稳定性.

结论:

开发的FTIR方法提供了一个快速,精确和准确的方法来量化Lycium barbarum中的LBPs.
这种方法可以作为一种有价值的补充或替代品,用于分析多糖的-硫酸测定.
这项研究为植物多糖的定量分析提供了一种新的策略.

关键词:

福里埃变换红外光谱法 (FTIR) 是一种方法.这种植物是Lycium barbarum.多糖类多糖化物.定量分析是一种量化分析.理论计算的理论计算.

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