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Updated: Sep 11, 2026

Efficient Generation of hiPSC Neural Lineage Specific Knockin Reporters Using the CRISPR/Cas9 and Cas9 Double Nickase System
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Efficient Generation of hiPSC Neural Lineage Specific Knockin Reporters Using the CRISPR/Cas9 and Cas9 Double Nickase System

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通过使用CRISPR/Cas9从H9获得的ID1/3淘汰赛人类胚胎干细胞系 (WAe009-A-2A和WAe009-A-2B) 的生成

Yihui Li1, Mingxia Du1, Zibing Jin1

  • 1Beijing Institute of Ophthalmology, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China.

Stem cell research
|September 29, 2024
PubMed
概括

研究人员使用CRISPR/Cas9.9生成了ID1/3淘汰赛人类胚胎干细胞系. 这些新型细胞系是研究DNA结合抑制剂 (ID) 蛋白在神经发育和心血管疾病中的作用的关键工具.

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CRISPR-Cas9 Mediated Gene Deletion in Human Pluripotent Stem Cells Cultured Under Feeder-Free Conditions04:21

CRISPR-Cas9 Mediated Gene Deletion in Human Pluripotent Stem Cells Cultured Under Feeder-Free Conditions

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相关概念视频

In-vitro Mutagenesis (Gene Knockouts)02:29

In-vitro Mutagenesis (Gene Knockouts)

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.