从Scutellaria baicalensis Georgi根中分离了十种黄类的制剂,使用二维逆流色谱与在线存储,稀释和混合接口
Lanjie Li1, Zhuo Liu2, Jinxing Guo2
1Shaanxi Key Laboratory of Natural Products & Chemical Biology, College of Chemistry & Pharmacy, Northwest A&F University, 22 Xiong Road, Yangling 712100, Shaanxi, China; College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, China.
概括
一个新的二维逆流色谱 (2D CCC) 系统与在线接口使自然产品的分离使用不同的溶剂系统. 这种先进的方法有效地从Scutellaria baicalensis根中分离出黄类.
科学领域:
- 自然产品化学 自然产品化学
- 染色学分离技术 染色学分离技术
- 植物化学 植物化学
背景情况:
- 反流色谱 (CCC) 通常需要多个溶剂系统来分离天然产品.
- 不兼容的溶剂系统阻碍了CCC中的在线二维连续分离.
- 需要一种新的方法来克服多维CCC中的溶剂不兼容性.
研究的目的:
- 开发一个在线的二维CCC系统,具有存储,稀释和混合的接口.
- 为了使不同溶剂系统在连续的2D CCC分离中能够使用.
- 应用开发的系统用于从Scutellaria baicalensis中分离的黄类药物.
主要方法:
- 设计了一个具有多功能在线接口的2D CCC系统.
- 该系统有助于在线储存,稀释和混合剂.
- 用于1D CCC的n-heptane-ethyl acetate-methanol-water (HepEMWat) 和用于2D CCC的乙烯酸乙烯-n-butanol-water (EBuWat) 的Scutellaria baicalensis黄的分离.
主要成果:
- 在1D CCC (正常模式) 中分离了6种脂性化合物.
- 在2D CCC (pH-峰值聚焦模式) 中分离了两个主要的亲水成分.
- 另外两种化合物通过随后的HPLC由于2D CCC中的共化而被净化.
结论:
- 开发的具有多功能接口的2D CCC系统对于天然产品净化非常有效.
- 这种方法克服了在线二维CCC中溶剂不兼容性的局限性.
- 该系统对复杂的天然产品混合物的高通量净化非常有希望.
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