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相关概念视频

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Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Updated: Jun 11, 2025

Isolating Human Peripheral Blood Mononuclear Cells and CD4+ T cells from Sézary Syndrome Patients for Transcriptomic Profiling
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在牛皮患者皮肤中使用单细胞RNA测序对长非编码RNA进行分析.

Rachael Bogle1, Matthew T Patrick1, Sutharzan Sreeskandarajan2

  • 1Department of Dermatology, University of Michigan Medical School, Ann Arbor, Michigan, USA.

The Journal of investigative dermatology
|September 29, 2024
PubMed
概括

在牛皮患者中,单细胞RNA测序成功地分析了数千种皮肤表达的长非编码RNA (lncRNAs). 这种方法确定了特定于细胞类型的lncRNA,并揭示了AC020916.16.

关键词:
生物信息学是一种生物信息学.角质细胞 (keratinocytes) 是一种细胞.这是一个LncRNARNA.牛皮是一种病.一个单细胞RNA-seqq.

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科学领域:

  • 皮肤病学 皮肤病学
  • 基因组学就是基因组学.
  • 分子生物学分子生物学

背景情况:

  • 长非编码RNAs (lncRNAs) 在表皮分化的作用是从大量RNA测序中知道的.
  • 大多数lncRNA缺乏单细胞水平的注释,尽管有组织特异性的表达.

研究的目的:

  • 评估单细胞RNA测序 (scRNA-seq) 用于对皮肤 lncRNAs进行分析.
  • 在牛皮皮肤中描述 lncRNA 和它们的细胞源.

主要方法:

  • 单细胞RNA测序 (scRNA-seq) 在6名患者的配对非参与性和病变性牛皮皮肤上.
  • 不同基因表达分析和空间转录组学验证.

主要成果:

  • 检测到超过7000种表达皮肤的lncRNA,并确定了它们的细胞起源.
  • 在病变性牛皮皮肤中鉴定了137个差异表达的lncRNAs.
  • 发现的角质细胞表达最差异表达的lncRNAs,包括AC020916.1,与增殖/分化基因相关.

结论:

  • scRNA-seq有效地对皮肤 lncRNAs 进行分析,并推断细胞起源.
  • 这种方法可以促进炎症性皮肤疾病的研究.