相关实验视频

Updated: Jun 13, 2025

High-resolution Respirometry to Measure Mitochondrial Function of Intact Beta Cells in the Presence of Natural Compounds

High-resolution Respirometry to Measure Mitochondrial Function of Intact Beta Cells in the Presence of Natural Compounds

Published on: January 23, 2018

miR-146a-5p调解炎症诱导的β细胞线粒体功能障碍和亡

Preethi Krishnan¹, Renato Chaves Souto Branco², Staci A Weaver³

¹Department of Medicine, Indiana University School of Medicine, Indianapolis, Indiana, USA; Herman B Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, Indiana, USA; Center for Diabetes and Metabolic Diseases, Indiana University School of Medicine, Indianapolis, Indiana, USA.

The Journal of biological chemistry

|September 29, 2024

概括

微RNA-146a-5p通过在炎症性压力期间损害线粒体功能来促进胰腺β细胞死亡和功能障碍,这是1型糖尿病的关键因素.

科学领域:

内分泌学在内分泌学.
分子生物学分子生物学
免疫学免疫学免疫学

背景情况:

在炎症期间,胰腺小岛的微RNA-146a-5p (miR-146a-5p) 升高调节.
过度表达miR-146a-5p与β细胞亡和减少胰岛素分泌有关.
精确的分子机制仍然不清楚.

研究的目的:

研究miR-146a-5p在胰腺β细胞功能和炎症条件下的生存中的作用.
阐明受β细胞中miR-146a-5p影响的分子通路.

主要方法:

开发出稳定的MIN6细胞系与miR-146a-5p过度表达或抑制.
用益炎性细胞因子 (IL-1β,IFNγ,TNFα) 治疗细胞以模仿体外1型糖尿病.
分析了细胞死亡,线粒体功能 (膜潜力,DNA拷贝数,呼吸,ATP),胰岛素分泌和基因表达 (RNA-seq).
从非肥胖糖尿病小鼠的小岛中检查了miR-146a-5p水平和线粒体标志物.

主要成果:

在炎症性压力下,miR-146a-5p过度表达增加了β细胞死亡和线粒体去极化.
抑制miR-146a-5p可以改善胰岛素分泌,线粒体DNA拷贝数,呼吸和ATP产生.
RNA-seq揭示了胰岛素分泌,亡和线粒体功能中的改变途径.
来自非肥胖糖尿病小鼠的小岛显示了miR-146a-5p的增加和线粒体功能标志物的减少.

结论:

在炎症性压力期间,miR-146a-5p促进胰腺β细胞功能障碍和亡.
抑制线粒体功能是miR-146a-5p发挥作用的关键机制.
miR-146a-5p是1型糖尿病的潜在治疗点.

关键词:

在 MIN6 单元中,设置 MIN6 单元.糖尿病糖尿病患者糖尿病患者在这种情况下,胰岛素胰岛素胰岛素.在 miR-146a-5p 中使用.这是一个微型RNA.线粒体功能线粒体功能胰腺小岛是什么意思一个β细胞β细胞.

更多相关视频

Isolated Pancreatic Islet Treatment and Apoptosis Measurement

Isolated Pancreatic Islet Treatment and Apoptosis Measurement

Published on: May 2, 2025

Author Spotlight: Assessment of Mitophagy Flux in Pancreatic β-Cells Using Effective and Robust Complementary Approaches

Author Spotlight: Assessment of Mitophagy Flux in Pancreatic β-Cells Using Effective and Robust Complementary Approaches

Published on: September 15, 2023

相关概念视频

The Intrinsic Apoptotic Pathway

The Intrinsic Apoptotic Pathway

Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...