Bmal1上调ATG5的表达,以促进皮肤皮肤黑色素瘤的自
Cellular signalling
|September 29, 2024
概括
皮肤皮肤黑色素瘤 (SKCM) 中Bmal1表达的减少与预后不佳相关,并影响瘤免疫微环境. Bmal1显示出作为SKCM的诊断和预后生物标志物的潜力.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 皮肤皮肤黑色素瘤 (SKCM) 是一种具有有限治疗选择的侵袭性癌症.
- 确定SKCM的新型治疗点至关重要.
- 在SKCM中,阿里尔碳化合物受体核转位器样 (ARNTL) 或Bmal1的作用尚不清楚.
研究的目的:
- 研究Bmal1在SKCM中的表达和功能.
- 确定Bmal1是否可以作为SKCM的诊断或预后生物标志物.
- 探索Bmal1与SKCM中的瘤免疫微环境 (TME) 之间的关系.
主要方法:
- 使用TCGA,GTEx,CCLE和ULCAN数据库进行Bmal1表达式分析.
- 通过CIBERSORT,R,TIMER,Sangerbox和Kaplan-Meier进行生存分析和免疫细胞透评估.
- 使用STRING和GSEA探索Bmal1-关联蛋白,并使用体外和体内SKCM模型进行补充.
主要成果:
- 与正常组织相比,SKCM中的Bmal1表达显著下降,与预后不佳相关.
- Bmal1的表达与免疫透有关,可能会影响TME,特别是瘤相关的中性粒细胞 (TAN).
- 过度表达Bmal1可以抑制黑色素瘤细胞的增殖和侵入,同时促进细胞亡,迁移和殖民地形成.
结论:
- Bmal1是SKCM诊断和预后的一个潜在的新生物标志物.
- Bmal1在调节SKCM进展和瘤免疫微环境方面发挥着重要作用.
- 准Bmal1可能为SKCM提供新的治疗策略.
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