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双S和六价对α-葡萄糖酶的联合作用:分子间相互作用,结构和功能变化

Jing Zhang¹, Honghui Wang¹, Yingmin Liao¹

¹Key Laboratory of Estuarine Ecological Security and Environmental Health (Fujian Province University), Tan Kah Kee College, Xiamen University, Zhangzhou 363105, PR China.

International journal of biological macromolecules

|September 29, 2024

概括

双S (BPS) 和六价 (Cr(VI)) 单独或一起破坏α-葡萄糖酶 (AG) 酶的结构和功能. 它们对AG的综合作用取决于暴露的顺序,影响碳水化合物代谢并造成健康风险.

科学领域:

环境化学环境化学
生物化学生物化学
毒理学毒理学毒理学

背景情况:

重金属和有机污染物的共同污染对健康构成重大风险.
双S (BPS) 和六价 (Cr(VI)) 是常见的环境污染物.
阿尔法-葡萄糖酶 (AG) 是碳水化合物代谢中的关键酶,使其成为有毒物质的潜在目标.

研究的目的:

为了研究BPS和Cr (VI) 与AG之间的分子间相互作用.
阐明BPS和CrVI单独和共存对AG结构和功能的影响.
了解联合毒性的分子机制.

主要方法:

在体外光谱技术 (如光火).
在Silico分子对接模拟中.
酶活性测定. 酶活性测定.

主要成果:

BPS和Cr(VI) 都与AG结合,通过静态和动态机制灭其光.
(VI) 显著改变了AG上的BPS结合部位,而BPS对 (VI) 结合部位的影响很小.
同时暴露于BPS和Cr(VI) 诱导AG的结构变化,其影响根据污染物添加的顺序而有所不同.
这两种污染物都表现出度依赖的AG活性抑制.

结论:

BPS和Cr(VI) 与AG相互作用,导致结构修改和功能损害.
暴露于BPS和Cr(VI) 的顺序影响它们对AG的联合毒性作用.
这项研究强调了与BPS和CrVI的同时暴露相关的潜在健康风险.

关键词:

阿尔法-葡萄糖化剂双 S 是一种双醇.酶活性的酶活动.六价的六价是一种分子间相互作用分子间相互作用结构性变化结构性变化

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