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开发和人类甲状腺有机体体外试验模型的初级特征,用于甲状腺代谢研究

Sofia Cristiani¹, Andrea Bertolini¹, Vittoria Carnicelli¹

¹Department of Surgical, Medical and Molecular Pathology and Critical Care Area, University of Pisa, Via Roma 55, 56126, Pisa, Italy.

Molecular and cellular endocrinology

|September 29, 2024

概括

一个新的3D甲状腺模型紧密模仿人体组织,提供更好的洞察力甲状腺功能和化合物效应. 这种先进的模型通过改善脱酶1 (IYD) 和代谢活性的研究来增强甲状腺研究.

科学领域:

内分泌学在内分泌学.
细胞生物学细胞生物学
生物技术是生物技术.

背景情况:

传统的二维细胞培养在准确建模复杂的甲状腺组织结构和功能方面存在局限性.
开发先进的体外模型对于了解甲状腺生理学和病理学至关重要.

研究的目的:

开发和描述一个3D甲状腺培养模型,更好地复制本地甲状腺组织.
在这个3D模型中,研究3-nitro-L-tyrosine (3-MNT) 对甲状腺神代酶1 (IYD) 活性和甲状腺特异性基因表达的影响.

主要方法:

开发一个3D甲状腺培养模型.
使用传输电子显微镜 (TEM) 和共聚焦显微镜进行形态分析.
基因和蛋白质表达分析通过qRT-PCR,免疫光学,西式涂抹,ELISA和LC-MS/MS.
评估甲状腺特异性标记物 (TPO,TSHR,PAX8,TTF-1,NIS,IYD,TG) 和代谢活动.

主要成果:

3D模型呈现出类似卵泡的结构,与本地甲状腺组织非常相似.
关键的甲状腺特异性基因和蛋白质标记物 (TPO,NIS,TG,TSHR,PAX8) 在3D培养中与2D培养相比显著上调.
3-MNT治疗抑制了IYD活性,由增加的MIT和DIT水平证明,并显著改变了NIS,TG,IYD,TSHR和TPO的表达.

结论:

开发的3D甲状腺模型有效地回顾了甲状腺组织的结构和功能特征.
这种3D模型是甲状腺研究,药物查和研究化合物对甲状腺功能影响的有价值和更具生理相关性的平台.

关键词:

这是一个3-MNT.干扰器是干扰器.这一天,我做了.有机器人机器人机器人机器人在 TSH 中,您可以使用甲状腺是什么?甲状腺是什么?

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