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Updated: Jun 11, 2025
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Preparation of Naringenin Solution for In Vivo Application
Published on: August 10, 2021
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在果叶中,naringenin石灰碳双键还原酶介导了二石灰生物合成
Yar-Khing Yauk1, Andrew P Dare1, Janine M Cooney2
1The New Zealand Institute for Plant and Food Research Limited (Plant and Food Research), Auckland 1142, New Zealand.
Plant physiology
|September 29, 2024
概括
研究人员确定了新的酶,纳灵宁缩酶 (NCRs),负责果中的二甲 (DHC) 生产. 这一发现允许对水果和其他应用中的DHC水平进行有针对性的操纵.
科学领域:
- 植物生物化学 植物生物化学
- 代谢工程是代谢工程.
- 果实质量 质量 果实质量
背景情况:
- 二甲基 (DHCs) 是果特有的黄类化合物,来自于烯胺路径.
- 之前关于DHC生物合成的假设,涉及Malus × domestica的基辛纳 CoA双键还原酶 (MdHCDBR),并没有得到实验证据的支持.
研究的目的:
- 为了确定负责果叶DHC生物合成的关键酶.
- 调查已识别的酶在DHC生产和代谢流量重定向中的作用.
主要方法:
- 进行比较转录组分析以确定候选基因.
- 在 *Nicotiana benthamiana* 中的过渡表达和转基因果系的产生.
- 酶活性测定和蛋白质局部化研究.
- 用于活动部位分析的AlphaFold建模.
主要成果:
- 在果叶中专门鉴定和表征了三种Malus × domestica naringenin chalcone reductase (MdNCR1a-c) 基因.
- MdNCR1基因对DHC产生至关重要,降低调节将叶子DHC减少85-95%.
- 减少DHC的产生将新陈代谢流转向了flavonol糖化物合成.
- MdNCR蛋白位在真空膜上,它们的活性部位对功能至关重要.
结论:
- 纳林基因缩酶 (NCRs) 是果DHC生物合成中缺失的酶.
- 了解NCR功能为操纵水果和其他生物技术应用中的DHC含量提供了途径.
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