在Monascus purpureus中对转录因子comp54181_c0的功能验证
Chan Zhang1,2,3, Haijiao Wang1, Arzugul Ablimit1
1School of Food and Health, Beijing Technology and Business University (BTBU), Beijing, China.
Journal of basic microbiology
|September 30, 2024
概括
转录因子comp54181_c0负面调节蒙纳科林K和Monascus中的色素生产. 它的缺失大大提高了这些有价值的化合物的生物合成.
科学领域:
- 微生物生物技术 微生物生物技术
- 分子生物学分子生物学
- 菌类的新陈代谢 菌类的新陈代谢
背景情况:
- 紫色 (Monascus purpureus) 通过发酵产生莫纳科林K,这是一个关键的他类药物前体.
- 控制莫纳科林K生物合成的调控机制尚未完全理解.
- 转录因子在控制真菌中二次代谢物产生的过程中起着至关重要的作用.
研究的目的:
- 为了研究转录因子comp54181_c0在Monascus中的功能.
- 阐明comp54181_c0在调节莫纳科林K和莫纳斯克色素生物合成中的作用.
- 提供对Monascus中二次代谢物产生的遗传调节的见解.
主要方法:
- 使用CRISPR/Cas9.0构建comp54181_c0的淘汰和过度表达菌株.
- 在工程菌株中对真菌形态,生长和代谢物产生进行比较分析.
- 实时光定量PCR (RT-qPCR) 用于评估基因转录水平.
主要成果:
- 干扰comp54181_c0导致真菌形态和生长的改变.
- 缺乏comp54181_c0显著增加了Monascus颜料和K. monacolin的生产.
- 发现comp54181_c0显著影响了参与莫纳科林K生物合成的基因的转录.
结论:
- 转录因子comp54181_c0在莫纳科林K和Monascus颜料的生物合成中起到负调节作用.
- 这项研究提高了对真菌二次代谢物调节的理解.
- 这些发现为通过基因操纵优化Monascus和Monacolin K生产提供了基础.
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