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CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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  1. 首页
  3. 基于crispr/cas系统的视觉检测方法的最新进展.
  1. 首页
  3. 基于crispr/cas系统的视觉检测方法的最新进展.

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DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning
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DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning

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基于CRISPR/Cas系统的视觉检测方法的最新进展.

Jinrong Chen1,2, Hang Su2, June Hyun Kim1

  • 1Department of Biotechnology, College of Engineering, The University of Suwon, Hwaseong 18323, Korea. liurcau@suwon.ac.kr.

Analytical methods : advancing methods and applications
|September 30, 2024

在PubMed 上查看摘要

概括
此摘要是机器生成的。

聚类定期间隔的短时间的平行体重复 (CRISPR) /关联核酶 (Cas) 提供快速,敏感的环境威胁视觉检测. 本综述探讨了基于CRISPR的诊断方法,重点是用于护理点应用的视觉读取.

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Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

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Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
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Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

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Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
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171

科学领域:

  • 分子诊断学 分子诊断学
  • 生物技术是生物技术.
  • 环境健康 环境健康

背景情况:

  • 传染病原体,毒素和重金属威胁全球健康和经济.
  • 迫切需要快速,灵敏和方便的视觉分子检测方法.
  • 克里斯普尔/卡斯系统已经成为分子诊断的强大工具.

研究的目的:

  • 审查基于CRISPR/Cas的视觉检测方法的最新发展.
  • 分析不同CRISPR/Cas效应器用于诊断的优缺点.
  • 讨论CRISPR/Cas视觉检测方面的挑战和未来前景.

主要方法:

  • 审查CRISPR/Cas效应器及其诊断原则.
  • 在临床检测 (POCT) 中专注于视觉读出信号.
  • 对色度,光和侧向流量测试的分析.

主要成果:

  • 克里斯普尔/卡斯技术使各种视觉检测策略成为可能.
  • 不同的CRISPR/Cas系统提供不同的诊断能力.
  • 视觉读取对于可访问的临床诊断至关重要.

结论:

  • 基于CRISPR/Cas的视觉检测对快速诊断具有重大前景.
  • 需要进一步的研究来克服当前的挑战和扩大应用.
  • 这些方法可以解决全球健康和环境监测需求.