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相关概念视频

Real Time RT-PCR02:57

Real Time RT-PCR

56.9K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
56.9K

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相关实验视频

Updated: Jun 11, 2025

Quantification of Circular RNAs Using Digital Droplet PCR
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Quantification of Circular RNAs Using Digital Droplet PCR

Published on: September 16, 2022

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评估RNA循环化效率的分析方法

Yali Sun1, Anis H Khimani1, Yanhong Tong1

  • 1Revvity Health Sciences, Revvity, Inc., Waltham, Massachusetts, USA.

Electrophoresis
|September 30, 2024
PubMed
概括
此摘要是机器生成的。

本研究引入了用于评估合成循环RNA (circRNA) 生产的新分析方法. 这些方法有助于研究人员优化circRNA载体工程,并了解RNA疗法的 ribozyme 催化活性.

关键词:
圆形的RNARNA是一个圆形的RNA.这是一个线性RNARNA.微流体毛细血管电泳矢量工程是指一个矢量工程.

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Identification of Circular RNAs using RNA Sequencing
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Discrimintion and Mapping of the Primary and Processed Transcripts in Maize Mitochondrion Using a Circular RT-PCR-based Strategy
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相关实验视频

Last Updated: Jun 11, 2025

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08:39

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Identification of Circular RNAs using RNA Sequencing
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Discrimintion and Mapping of the Primary and Processed Transcripts in Maize Mitochondrion Using a Circular RT-PCR-based Strategy
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科学领域:

  • 生物化学和分子生物学
  • 在RNA治疗方面,RNA疗法.
  • 合成生物学 合成生物学

背景情况:

  • 循环RNA (circRNAs) 由于其稳定,闭环结构,在RNA治疗中至关重要.
  • 基于 ribozyme 的策略在合成 circRNA 生产中很常见.
  • 目前的分析方法缺乏 circRNA 矢量工程的高通量和定量能力.

研究的目的:

  • 开发和详细分析方法来表征基于 ribozyme 的 RNA 循环化.
  • 为了使 circRNA 合成效率的高通量和定量评估.
  • 支持优化circRNA矢量工程和 ribozyme 催化活性.

主要方法:

  • 详细描述用于circRNA表征的分析方法.
  • 基于 ribozyme 的 RNA 循环化效率的评估.
  • 专注于定量和高通量分析.

主要成果:

  • 建立了可靠的分析方法来评估circRNA循环化.
  • 为合成circRNA生产提供了定量评估的框架.
  • 在优化 ribozyme 中介循环化的过程中证明了效用.

结论:

  • 开发的分析方法对于推进合成circRNA生产至关重要.
  • 这些方法有助于高效的circRNA矢量工程和优化.
  • 该研究通过改进合成RNA生产分析,为RNA治疗领域做出了贡献.