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在Pristionchus的发育转录学揭示了可塑性基因调节网络的逻辑

Shelley Reich¹, Tobias Loschko², Julie Jung¹

¹School of Biological Sciences, University of Utah; Salt Lake City, Utah, USA.

bioRxiv : the preprint server for biology

|September 30, 2024

概括

线虫的口腔形状可塑性是由双层遗传逻辑控制的. 两个关键的基因,eud-1和seud-1/sult-1,在关键的发育窗口中作为顺序检查点.

科学领域:

发育生物学是发展生物学.
进化遗传学的进化遗传学
尼马生物学的生物学

背景情况:

发育性可塑性允许有机体在响应环境线索时产生不同的表型.
了解可塑性的遗传基础,特别是线虫中的口型可塑性,仍然是一个挑战.
之前的基因查发现了30个基因,这些基因影响了Pristionchus pacificus*的口腔塑性.

研究的目的:

阐明控制口腔形状可塑性的遗传调节逻辑.
在环境敏感性关键窗口期间调查基因表达的时间动态.
为了确定关键的基因和途径,参与整合环境信息的发展决策.

主要方法:

构建一个基因调节网络 (GRN) 用于口腔塑性.
在各种环境条件,遗传背景和口型突变物中执行发育转录学.
分析基因表达模式,以评估监管逻辑和识别关键基因.

主要成果:

只有两个基因,eud-1*和seud-1/sult-1*,在关键窗口期间对环境敏感,作为顺序检查点.
在GRN中发生的突变显示出时间约束,并且观察到口型基因之间的反循环.
*seud-1/sult-1*表达与口腔形状在各种菌株和物种的变化相关.
代谢被确定为一种共同的途径,调节口腔塑性.

结论:

*Pristionchus pacificus*的口腔形状可塑性是由一个受约束的两层遗传逻辑调节的.
这些发现揭示了在开发过程中整合环境信号的新机制.
一个闪亮的应用程序被开发,以促进多种条件的基因表达的比较分析.

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