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颗粒状后皮质的独特的转录基因细胞类型在小鼠和老鼠中被保存,尽管关键标记基因发生了剧烈变化
Isla A W Brooks1, Izabela Jedrasiak-Cape1, Chloe Rybicki-Kler1,2
1Dept. of Psychology, University of Michigan, Ann Arbor, MI 48109.
bioRxiv : the preprint server for biology
|September 30, 2024
概括
鼠和老鼠的颗粒状后皮层 (RSG) 分享保存的细胞类型,包括低基神经元 (LR) 和金字塔细胞. 然而,物种特异性标记基因需要仔细考虑转基因谱系的发展.
科学领域:
- 神经科学是一个神经科学.
- 比较解剖学的比较.
- 分子生物学分子生物学
背景情况:
- 颗粒状后皮质 (RSG) 对于记忆和空间导航至关重要.
- 鼠标RSG表现出独特的细胞类型,例如低基神经元 (L2/3 LR) 和特定的金字塔细胞 (L5a RSG).
- 在小鼠和老鼠之间,这些独特的细胞类型的进化保存是未知的.
研究的目的:
- 研究小鼠和老鼠之间的颗粒状回质皮质 (RSG) 中的转录组独特细胞类型的进化保存.
- 确定跨物种标记基因,以准小鼠和大鼠的特定RSG细胞类型.
主要方法:
- 对老鼠和老鼠RSG进行比较的转录基因分析.
- 细胞类型特定标记基因的识别和验证.
- 对雄性和雌性动物的基因表达模式的分析.
主要成果:
- 鼠和老鼠RSG共享同源细胞类型,一些亚型如L2/3LR和L5aRSG神经元在老鼠中更为丰富.
- 关键的细胞类型特异性标记基因,如小鼠L5a RSG神经元中的Scnn1a,在老鼠中没有保存.
- 确定了替代的跨物种标记基因,用于针对两种物种中的RSG细胞类型.
结论:
- 颗粒状后皮质 (RSG) 独特的细胞类型在小鼠和老鼠之间在进化过程中得到保护.
- 标记基因表达存在显著的特定物种差异,影响转基因模型的发展.
- 已识别的跨物种标记物有助于针对性地操纵小鼠和大鼠的RSG细胞类型.
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