通过ERECTA的切换开关机制,防止不适当的信号在结合前和结合后的感知
Liangliang Chen1,2,3, Michal Maes1,3, Alicia M Cochran1
1Howard Hughes Medical Institute, The University of Texas at Austin, Austin, TX, 78712 USA.
阿拉比多的ERECTA (ER) 受体激酶活性是由其C端尾部 (ER_CT) 控制的. 这个尾巴充当光开关,调节受体信号传递,并确保在连接体感知时暂时激活.
科学领域:
- 植物分子生物学 植物分子生物学
- 受体激酶的信号传递.
- 信号传导机制的信号传导机制.
背景情况:
- 信号事件的动态控制依赖于快速受体调节.
- 阿拉比多普西斯的ERECTA (ER) 受体激酶通过感知联体来控制植物的发育.
研究的目的:
- 为了阐明控制不适当的阿拉比多普西斯ERECTA (ER) 受体激酶活性的机制.
- 确定ER C-终端尾部 (ER_CT) 如何调节ER 激酶功能和信号传递.
主要方法:
- 研究了ER C端尾 (ER_CT) 在受体自身抑制中的作用.
- 确定了ER_CT的结合伙伴,包括BKI1和E3酶PUB30/PUB31.
- 通过共同受体BAK1.1,确定ER_CT的酸化状态.
主要成果:
- 移除ER C-终端尾部 (ER_CT) 会导致ER 激酶活性和过活性的增加.
- ER_CT对于结合抑制剂BKI1和E3化酶PUB30/PUB31.1至关重要.
- BAK1对ER_CT的酸化释放了自身抑制,调节了BKI1和PUB30/31的相互作用.
结论:
- ER C-终端尾部 (ER_CT) 作为光开关,作为一个关闭-启动-关闭开关.
- 这种机制保持了基底ER活动的低水平,并确保在连接体感知时传递短暂,强大的信号.
- 阐明了一种用于微调植物受体激酶信号的新型光开关模块.
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