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Updated: Jun 11, 2025

CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery

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定制基于CRISPR/Cas的表观基因组编辑器,用于可编程的染色体化和降低细胞毒性

Jacob Goell¹, Jing Li¹, Barun Mahata¹

¹Department of Bioengineering, Rice University, Houston, TX 77030, USA.

bioRxiv : the preprint server for biology

|September 30, 2024

概括

研究人员设计了酶以进行精确的表观基因组编辑,从而实现向基因激活和改善治疗交付. 这一进步为理解基因调节和开发功能性基因组选提供了新的工具.

科学领域:

表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
分子生物学分子生物学
合成生物学合成生物学

背景情况:

基因酸酶调节基因表达和细胞过程.
目前的表观基因组编辑工具在精度和交付方面存在局限性.

研究的目的:

开发工程化氨酸转移酶,用于可编程的基因组酸转化.
为了创建人类p300的少细胞毒性变体,以改善病毒传递.
利用这些工具进行功能性基因组查和增强器-基因映射.

主要方法:

氨酸转移酶的工程用于向乙化和克罗化.
人类p300的突变以减少细胞毒性,同时保持酶活性.
单细胞CRISPR激活查用于增强剂基因映射.
研究化特异性相互作用和p300招募对主要编辑的影响.

主要成果:

工程酶使得可以编程的基因素化沉积成为可能.
用工程crotonyltransferase向促进体,可以激活内源基因.
较少的细胞毒性p300变种可以改善病毒传递.
通过单细胞CRISPR查,成功地绘制增强剂的地图,以在现场针对基因.
p300招募提高了主要编辑效率,独立于其催化活动.

结论:

开发了新的可编程表观基因组编辑工具.
在表观遗传学中扩大了对 lysine acylation 在表观遗传学中的机械作用的理解.
实现了功能性基因组选和治疗开发的新方法.

关键词:

克里斯普尔是什么意思?克里斯普尔是什么意思?克里斯普拉是一个.EP300 EP300 在线播放在表观基因组编辑.这就是 Perturb-seqq.增强剂是一种增强剂.基因素乙化基因素乙化基因素乙化组织基因子的克罗托尼化.在目标之外编辑.蛋白质工程工程蛋白质工程

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