超声波产生的泡增强了复合原体水凝中中介细胞 stromal 细胞的骨质分化
Somnath Maji1, Mitra Aliabouzar1,2, Carole Quesada1
1Department of Radiology, University of Michigan, Ann Arbor, MI, USA.
Bioactive materials
|September 30, 2024
概括
响应超声波的水凝使用相位变异乳液,以非侵入性地控制介酶体 stromal 细胞 (MSC) 的分化. 这项技术使生物物理线索的按需调制成为可能,为再生医学应用指导细胞命运.
科学领域:
- 生物材料科学 生物材料科学
- 再生医学是一种再生医学.
- 细胞生物学 细胞生物学
背景情况:
- 水凝对于传递介质细胞 stromal 细胞 (MSCs) 至关重要,模仿细胞外基质.
- 目前的水凝缺乏对生物物理线索的动态控制,限制了指向的MSC差异化.
- 刚性和粘弹性是影响MSC差异化的关键因素.
研究的目的:
- 开发一种新的复合水凝,用于非侵入性,超声波触发的MSC骨质分化的调制.
- 研究超声波诱导泡形成对水凝特性和MSC行为的影响.
- 评估在3D环境中对细胞分化的按需控制的潜力.
主要方法:
- 使用I型原蛋白和相位移乳液制造复合水凝.
- 应用超声波来诱导乳液的非热蒸发,产生气泡.
- 使用Young的模量测量,对水凝硬化和泡周围的紧缩进行了表征.
- 在体外和体内评估MSC活力,增殖和骨质分化标志物 (RUNX2,骨素,性酸酶,沉积).
- 评估MSC茎度标志物 (CD90) 和植入水凝的组织学分析.
主要成果:
- 超声波暴露诱导了泡形成,导致局部矩阵紧缩和泡附近的硬度增加了2.5倍.
- MSC的生存能力和扩散不受泡形成的影响.
- 在水凝中封装的MSC显示在气泡附近显著增强了骨质分化标志物 (RUNX2,骨质) 和活性 (ALP,沉积).
- 在泡附近,MSC茎度标记物 (CD90) 的表达减少.
- 在体内研究显示持续的泡,局部的原蛋白对齐和增加的核不对称.
结论:
- 开发的复合水凝允许对MSC骨质分化的非侵入性,超声中介控制.
- 超声波诱导的水凝刚性的局部变化可以指导细胞命运.
- 这项技术为再生医学中的按需3D细胞分化提供了一个有前途的平台.
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