具有成本效益的染色替代品,以免疫组织化学的氨酸演示在口腔上皮层病理
Himanta Ghritlahare1, A Einstein2, Swatantra Patel3
1Oral Pathology and Microbiology, Government Dental College, Raipur, Chhattisgarh, India.
Journal of pharmacy & bioallied sciences
|September 30, 2024
概括
常规组织学污点,如H和E,mPap,AS和DH,有效地检测皮质组织中的角质素. 艾尤布-什克拉尔 (AS) 染色体表现出检测质素的最高特异性和强度.
科学领域:
- 组织病理学 组织病理学
- 口腔病理学 口腔病理学
- 生物标志物检测检测 生物标志物检测
背景情况:
- 质蛋白检测对于诊断上皮质病理至关重要.
- 质素的免疫组织化学 (IHC) 染色是特定的,但成本昂贵且耗时.
- 开发具有成本效益的组织学方法来检测质蛋白对于常规实验室来说很重要.
研究的目的:
- 评估常见的组织学染色剂在正常口腔粘膜,高甲状腺瘤和差异化状细胞癌中检测质素的疗效.
- 为了比较不同常规组织学方法之间的质素染色的特异性和强度.
主要方法:
- 五种组织学染色 (H和E,mPap,AS,DH,AB-PAS) 应用于甲固定,嵌的口腔组织样本 (正常的粘膜,高正,WDSCC).
- 染色部分被两个独立观察员评估,以测量质染的特异性和强度.
- 进行了统计分析,以比较不同方法的染色特性.
主要成果:
- 所有五种测试染色,包括H和E,mPap,AS,DH和AB-PAS,在所有组织类型中都显示出阳性质染色.
- 阿尤布-什克拉尔 (AS) 染料在特异性和强度方面获得了最高分.
- 在所有评估的组织中,Alcian蓝色-PAS (AB-PAS) 染料的染色强度是最低的.
结论:
- 常规染色,如H和E,mPap,AS和DH是有效的检测质蛋白在正常的口腔粘膜,多直角质症和WDSCC.
- AS染色是一种高效和特定的方法,用于在这些组织中常规检测质素.
- 与其他方法相比,AB-PAS对角质蛋白的染色不那么强烈,可能不太适合常规诊断目的.
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