在模型微生物中的杀虫剂诱导的代谢失调E.E. 通过综合代谢分析 (Comprehensive Metabolic Profiling) 发现的
Chao Guo1, Li Chen1,2, Rui Xu1
1Human Nutrition Program, Department of Human Sciences, The Ohio State University, Columbus, Ohio 43210, United States.
ACS omega
|September 30, 2024
概括
常见的杀虫剂如fipronil和permethrin可以被大肠杆菌降解,但马拉却不能. 暴露于杀虫剂会改变肠道细菌的新陈代谢,影响氨基酸通路和潜在的宿主健康.
科学领域:
- 环境科学 环境科学
- 微生物学 微生物学
- 毒理学 毒理学 毒理学
背景情况:
- 广泛使用的杀虫剂包括fipronil,malathion和permethrin,导致残留量超过安全水平.
- 接触这些杀虫剂会带来危险,包括神经毒性,器官损伤和肠道微生物群失生症.
- 这些杀虫剂对肠道细菌代谢和宿主健康的影响尚不清楚.
研究的目的:
- 调查菲普罗尼尔,马拉和对大肠杆菌 (Escherichia coli) 的生长和新陈代谢的影响,这是一种代表性肠道细菌.
- 为了确定大肠杆菌是否可以生物降解这些常见的杀虫剂.
- 为了确定受E. coli在杀虫剂暴露影响的代谢途径.
主要方法:
- 对非致病性大肠杆菌暴露于fipronil,malation和perethrin的生理学相关度.
- 监测细菌生长抑制.
- 利用代谢学和代谢途径分析来评估细菌代谢的变化.
主要成果:
- 菲普罗尼尔,马拉和甲显著抑制了大肠杆菌的生长.
- 大肠杆菌表明了fipronil和的生物降解,但不是马拉.
- 暴露在杀虫剂中导致大肠杆菌的代谢概况发生了化学依赖的变化,特别是影响了氨基酸代谢途径.
结论:
- 大肠杆菌可以生物降解菲尼尔和甲酸,有助于从肠道环境中去除它们.
- 马拉对大肠杆菌的生物降解具有抗性.
- 杀虫剂在肠道细菌中诱导显著的化学特异性代谢干扰,对氨基酸代谢产生显著影响,可能影响宿主健康.
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