Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

9.1K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
9.1K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

16.0K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
16.0K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Fragmented sleep during pregnancy induces inflammation and tryptophan-kynurenine pathway metabolism: Importance of elevated fetal brain kynurenic acid.

bioRxiv : the preprint server for biology·2026
Same author

Multispectral coded light for high-speed temperature imaging of aluminum combustion.

Optics express·2026
Same author

Biochemical Regulation of Brain Kynurenic Acid Synthesis and Inhibition in Rats Is Sensitive to the Time of Day.

ACS chemical neuroscience·2026
Same author

Liquid volume fraction and droplet sizing in atomizing sprays using polarization ratio with dual structured laser illumination planar imaging: erratum.

Optics letters·2026
Same author

The DNA/RNA autophagy protein SIDT2 as a novel neuropathological hallmark in Huntington disease.

Brain pathology (Zurich, Switzerland)·2026
Same author

Biochemical Regulation of Brain Kynurenic Acid Synthesis and Inhibition in Rats is Sensitive to the Time of Day.

bioRxiv : the preprint server for biology·2026

相关实验视频

Updated: May 4, 2026

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
08:53

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

9.7K

用于SILMAS的图像后处理:结构化照明光板显微镜与轴向扫描.

David Frantz1, Courtney J Wright2, Allison J Schaser3

  • 1Division of Combustion Physics, Department of Physics, Lund University, Lund, Sweden.

Biomedical optics express
|September 30, 2024
PubMed
概括

我们开发了一种后处理方法,以减少扫描光板显微镜 (SILMAS) 数据中的文物. 该技术提高了清除组织体积成像的图像质量,改善了定量分析.

更多相关视频

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

25.1K
Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

9.8K

相关实验视频

Last Updated: May 4, 2026

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
08:53

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

9.7K
A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

25.1K
Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

9.8K

科学领域:

  • 生物医学成像技术 生物医学成像技术
  • 显微镜技术 显微镜技术
  • 计算成像技术的成像

背景情况:

  • 像SILMAS这样的体积显微镜技术提供了改进的对比度和光学切割.
  • 在SILMAS中,高效的散射光去除可以突出像不均性,阴影和信号衰减这样的工件.

研究的目的:

  • 提出和验证SILMAS数据中文物抑制的后处理方案.
  • 加强从生物样本体积显微镜提取定量数据.

主要方法:

  • 应用了一个后处理方案,将Beer-Lambert定律用于光衰减补偿和用于遮影条纹抑制的计算策略.
  • 将额外的带通步骤纳入波纹-里埃过中,用于在不引入新扭曲的情况下删除工件.
  • 展示了对α-synuclein转基因小鼠大脑数据的技术.

主要成果:

  • 在SILMAS体积数据中成功抑制了照明不均性,阴影条纹和信号衰减.
  • 实现了对光衰减的近量化补偿.
  • 过的高对比度光片显微镜数据具有最小的扭曲和没有新的文物.

结论:

  • 拟议的后处理方案显著提高了SILMAS体积数据的统一性和质量.
  • 该技术适用于成像清除的组织样本,直至厘米尺度,以微米级的同位素分辨率.
  • 这种人工物抑制方法使体积显微镜中更可靠的定量分析成为可能.