动态多谱NIR/SWIR用于in vivo淋巴血管结构和功能量化
Christopher Hansen1, Jaidip Jagtap2, Abdul Parchur3
1Medical College of Wisconsin, Department of Biomedical Engineering, Milwaukee, Wisconsin, United States.
Journal of biomedical optics
|September 30, 2024
概括
与近红外第一窗 (NIR-I) 成像相比,短波红外 (SWIR) 成像提供了优越的淋巴血管可视化. 这种先进的技术增强了淋巴循环评估,提高了分辨率和灵敏度.
科学领域:
- 生物医学成像技术 生物医学成像技术
- 淋巴系统研究 淋巴系统研究
- 光学成像技术的技术.
背景情况:
- 淋巴系统在液体平衡和免疫力中的关键作用受到有限的功能性表征方法的阻碍.
- 目前的成像技术在清晰度和对比度方面扎,以进行细致的淋巴血管分析.
研究的目的:
- 调查短波红外 (SWIR) 光成像对*in vivo*淋巴结构和功能评估的有用性.
- 为了比较SWIR成像与常规的近红外第一窗口 (NIR-I) 成像用于淋巴视觉化.
主要方法:
- 使用NIR-I (700-900 nm) 和SWIR (900-1800 nm) 光成像,评估了斯普拉格-道利大鼠的地下淋巴循环.
- 在皮肤内注射脚后,对SWIR淋巴成像的对比剂中,比较了绿 (ICG) 和硫化银 (QD) 量子点作为对比剂.
主要成果:
- 与NIR-I相比,SWIR成像显示了减少的散射和自发光.
- 使用ICG的SWIR成像显示了比NIR-I更好的1.7倍的分辨率和灵敏度.
- 使用QD的SWIR成像实现了近2倍的更好的分辨率和灵敏度,增强了船只的辨别能力.
结论:
- 与NIR-I相比,SWIR成像为淋巴血管可视化提供了更高的分辨率和灵敏度.
- 在SWIR成像中,硫化银QD提供了比NIR-I中的ICG更强大的淋巴成像能力.
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