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在非洲肺鱼,Protopterus annectensens的嗅觉器官中的2型鼻受体表达

Shoko Nakamuta¹, Zicong Zhang², Masato Nikaido³

¹Laboratory of Veterinary Anatomy, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka, Iwate, 020-8550, Japan.

Cell and tissue research

|September 30, 2024

概括

肺鱼的嗅觉器官显示中间V2R表达模式,桥梁鱼和四足动物的嗅觉受体分离. 这表明,在主要嗅觉上皮和鼻器官的发展中,这是一个关键的进化步骤.

科学领域:

嗅觉系统的演变嗅觉系统的演变
进行比较的神经解剖学.
分子生物学分子生物学

背景情况:

四足动物拥有主要的嗅觉上皮 (OE) 和辅助的口鼻器官 (VNO).
远鱼缺少VNO,而肺鱼有一个状OE和类似于VNO的内腔表皮 (RecE).
假设ReceE是VNO的前体,其V2R表达模式不清楚.

研究的目的:

为了研究肺鱼 (Protopterus annectens) 片状OE和ReceE2型鼻受体 (V2Rs) 的表达模式.
为了确定V2R表达在肺鱼的这两个嗅觉结构之间是否差异分布.

主要方法:

在Protopterus annectens的叶片OE和Rece中分析V2R基因表达.
根据它们的表达部位 (仅OE,仅Rece或两者) 来对已识别的V2R进行分类.
对肺鱼V2Rs的遗传学分析,以了解它们的进化关系.

主要成果:

肺鱼的V2R被分为三个表达群:仅限于状OE,仅限于Rece,并且在两者中都存在.
仅在 RecE 中的 V2R 和在 OE 和 RecE 中的 V2R 形成了一个独特的遗传分类.
仅表达在叶片OE中的V2Rs构成了单独的组.

结论:

肺鱼的V2R表达模式是中间的,显示片状OE和Rece之间的部分分离.
这些发现支持了ReceE作为原始VNO结构的假设.
这项研究揭示了四足动物嗅觉受体表达的分歧的关键进化步骤.

关键词:

进化进化进化进化进化进化进化在现场混合化.肺鱼是一种肺鱼.在RNA-seqqq.吐口鼻子器官是什么嘔吐鼻腔受體的使用

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