水溶性维生素 稳定性通过坚固的液体染色学-质谱学
Wei Luo1, Danchen Wang1, Yueming Tang1
1Department of Laboratory Medicine, Peking Union Medical College Hospital, Peking Union Chinese Academy of Medical Sciences, Beijing, China.
Annals of nutrition & metabolism
|September 30, 2024
概括
水溶性维生素的稳定性因储存条件而异. 为了进行准确的实验室检测,维生素如胺和甲酸需要冷储存 (低至80°C长达7天),而冰浴则可以在分析前保存全血样本.
科学领域:
- 临床化学 临床化学
- 生物化学 生物化学
- 分析化学 分析化学
背景情况:
- 精确测量水溶性维生素对于诊断缺陷至关重要.
- 确定维生素稳定性极限对于可靠的实验室测试至关重要.
- 本研究评估了水溶性维生素在各种储存条件下的稳定性.
研究的目的:
- 为了确定通常测量的水溶性维生素的稳定性.
- 评估不同储存温度和时间对维生素稳定性的影响.
- 优化分析前条件,以准确确定维生素水平.
主要方法:
- 分析了胺,利博弗拉,尼古丁胺,潘托酸,氧化酸/氧化酸,生物素,5-甲基四基叶酸 (5-MTHF) 和酸的稳定性.
- 利用液体染色学-双重质谱仪进行精确量化.
- 储存温度,时间,样品类型 (血清/血) 和冰浴前离心的研究影响.
主要成果:
- 胺,甲酸和甲酸在室温和2-8°C的血清中显示不稳定.
- 血清中的 рибофлавин和5-MTHF 在2-8°C下分别稳定至48h和72h.
- -20°C的储存保证了长达72小时的稳定性; -80°C的储存保证了所有测试维生素长达7天的稳定性.
- 除了尼古丁胺,全血中的维生素在冰浴中稳定了2-4小时.
结论:
- 在水中溶解的维生素 (例如,硫胺, рибофлавин,氧化,酸) 在环境和冷藏温度下不稳定.
- 在-80°C的最佳储存确保了长达7天的稳定性.
- 冰浴提高了离心前全血样本的稳定性,强调了在维生素检测中需要严格的分析前质量控制的需要.
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