来自Monascus pilosus的水溶性菌多糖:提取,结构特征,免疫调节效果和产量通过过度表达UGE基因而增强
Fanyu Ye1, Yajuan Chen1, Jun Liu1
1National Engineering Research Center of Rice and Byproduct Deep Processing, Central South University of Forestry and Technology, Changsha, Hunan 410004, China.
International journal of biological macromolecules
|September 30, 2024
概括
这项研究从Monascus pilosus中分离出一种强效的菌体多糖化物 (MPP-0.1),显示出显著的免疫调节作用和增强RAW264.7细胞活性. 在M. pilosus中过度表达UGE基因显著增加了MPP生产和提取产量.
科学领域:
- 菌类学 菌类学是指菌类学.
- 生物化学 生物化学
- 免疫学 免疫学 免疫学
背景情况:
- 菌多糖 (MPP) 呈现出多样化的生物活动.
- 莫纳斯克 (Monascus pilosus) 是一种真菌物种,以产生生物活性化合物而闻名.
- 了解MPP的结构和功能对于开发新疗法至关重要.
研究的目的:
- 从Monascus pilosus中分离和鉴定细胞多糖 (MPP) 的特征.
- 为了研究纯化的MPP分量的免疫调节作用.
- 通过基因工程增强MPP生产.
主要方法:
- 性提取和MPP分量的净化.
- 分子重量测定,热稳定性分析和糖结合模式阐明.
- 在体外评估免疫调节活性使用RAW264.7细胞和促炎细胞因子分析.
- 通过过度表达UDP-glucose 4-epimerase (UGE) 基因,对M. pilosus进行遗传修饰.
主要成果:
- MPP-0.1分离显示出更高的纯度和产量,分子量为3.7776 × 104 Da,热稳定性高达170 °C.
- MPP-0.1表现出显著的免疫调节作用,包括增强氧化,TNF-α,IL-6和IL-1β的产生,并增加了细胞活性.
- 过度表达UGE基因导致M. pilosus生物量增加62.56%和MPP提取产量增加83.19%.
结论:
- MPP-0.1具有显著的免疫调节特性,使其成为治疗应用的潜在候选者.
- 通过UGE基因过度表达对M. pilosus的基因工程是一种有效的策略,可以增强MPP的产生.
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