非编码地RNA1-1通过与CUG结合蛋白1相互作用,损害肠上皮膜更新和屏障功能
Shweta Sharma1, Lan Xiao2, Hee K Chung1
1Cell Biology Group, Department of Surgery, University of Maryland School of Medicine, Baltimore, Maryland.
细胞外囊中的高RNA1-1 (vtRNA1-1) 会损害肠道屏障功能和上皮的更新. 这项研究揭示了vtRNA1-11-1.
科学领域:
- 分子生物学分子生物学
- 胃肠病学 胃肠病学
- 细胞生物学 细胞生物学
背景情况:
- 小型非编码RNAs (vtRNAs) 参与细胞过程,但它们在肠表皮中的作用尚不清楚.
- Vault RNA 1-1 (vtRNA1-1) 是一种特定的vtRNA,在健康和疾病中具有潜在的作用.
研究的目的:
- 研究人类vtRNA1-1在肠道上皮质更新和屏障完整性中的功能.
- 阐明vtRNA1-1影响肠道粘膜的机制.
主要方法:
- 研究使用了vtRNA1-1转基因小鼠,初级肠细胞和Caco-2细胞.
- 从休克患者和败血症小鼠血清中分离出细胞外囊泡 (EVs).
- 肠道器官 (肠道器官) 用于通过FITC-dextran和Ki67免疫染色等测试来评估粘膜生长和屏障功能.
主要成果:
- 在休克患者和败血症小鼠的EV中发现vtRNA水平升高,与粘膜损伤和屏障功能障碍相关.
- 转基因vtRNA1-1在小鼠中的表达减少了粘膜生长,帕内斯细胞数量和细胞间结合蛋白,增加了肠道的脆弱性.
- 在体外,静止vtRNA1-1改善了屏障功能,而富含vtRNA1-1的EVs恶化了细胞透性.
结论:
- 在EV和粘膜组织中增加的vtRNA1-1抑制了肠上皮的更新和屏障功能.
- 对vtRNA1-1/CUGBP1轴的调节失调有助于严重疾病中肠道粘膜的破坏.
- 这项研究确定了一种新的途径,在危急疾病期间影响肠道健康.
更多相关视频
12:15The C. elegans Intestine As a Model for Intercellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis at the Single-cell Level: Labeling by Antibody Staining, RNAi Loss-of-function Analy
Published on: October 3, 2017
07:42Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses
Published on: July 13, 2016
相关概念视频
RNA Editing
Mucosal Barrier of the Stomach
Within parietal cells, carbonic acid is first formed through the reaction of water and carbon dioxide. The dissociation of carbonic acid releases bicarbonate and hydrogen ions. The bicarbonate...
Regulation of Nuclear Protein Sorting
Role Of Notch Signalling In Intestinal Stem Cell Renewal
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Tight Junctions
Nuclear Export of mRNA
