双离体内基聚基解形状揭示了泛癌转录组中的多方面的基因功能
Jiao Sun1,2, Jin-Young Kim3, Semo Jun4
1Department of Computer Science, University of Central Florida, Orlando, FL, 32816, USA.
Experimental & molecular medicine
|September 30, 2024
概括
在内子 (内子APA) 中的替代裂变和多化产生了不同的mRNA异型. 这项研究揭示了正常和癌症组织中的内基APA模式,影响蛋白质结构和功能.
科学领域:
- 分子生物学分子生物学
- 基因组学就是基因组学.
- 癌症研究 癌症研究
背景情况:
- 在内子 (intronic APA) 中的替代裂变和多化产生较短的mRNA异型,其生理作用不清楚.
- 哺乳动物的拉巴胺素 (mTOR) 途径的标调节基因表达,但其对内部APA的影响尚未完全理解.
研究的目的:
- 开发一个工作流程来分析内部APA配置文件.
- 调查mTOR活动对内部APA的影响.
- 探索内部APA在正常和癌症转录组中的作用及其对蛋白质组编程的影响.
主要方法:
- 开发了一个全面的工作流程来分析内部APA配置文件.
- 利用mTOR调节的转录组作为一个模型系统.
- 将工作流应用于来自癌症基因组图谱 (TCGA) 的RNA-seq数据.
- 分析了因内部APA事件导致的蛋白质长度变化和C端变化.
主要成果:
- 鉴定了对应于mTOR活动的对比的内部APA模式 (增加或减少).
- 证明二分体内基APA模式在正常和癌症组织中保持.
- 揭示了两个不同的蛋白质组编程现象:功能域的损失或最小的C端变化.
- 观察到癌症转录组中最小的C端变异的丰富,以及正常组织中功能域的丧失.
- 在JMJD6中展示了异形特异性的功能差异,这些差异是由非结构化的C终端区域的内在APA驱动的.
结论:
- 内部APA事件代表了正常和癌症转录组中的分子特征.
- 在癌症中,Intronic APA有助于蛋白质多样性和功能调节.
- 由于内在的APA导致非结构化C端蛋白区域的变化可能导致功能分歧.
相关概念视频
02:39lncRNA - Long Non-coding RNAs
8.5K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.5K
01:37Epigenetic Regulation
3.0K
Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
X-chromosome...
3.0K
03:18Non-LTR Retrotransposons
11.4K
As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
11.4K