梅拉德反应产物通过调节洗肌肉模型中的肌球蛋白稳定性来抑制脂质氧化
Jiarong Cao1, Haixia Yan1, Yixiao Shen1
1College of Food Science, Shenyang Agricultural University, Shenyang, Liaoning, China.
Journal of food science
|September 30, 2024
概括
梅拉德反应产物 (MRPs) 能够有效地抑制肌球蛋白氧化,这是鱼类肌肉中脂质氧化的关键驱动因素. 这种抗氧化作用保留了鱼的质地和质量,延长了保质期.
科学领域:
- 食品科学 食品科学 食品科学
- 生物化学 生化学
- 肌肉生物学 肌肉生物学
背景情况:
- 脂质氧化是肌肉恶化的主要原因之一.
- 血红蛋白的氧化,特别是肌球蛋白 (Mb) 的氧化,对肉类中的脂质氧化有显著的贡献.
- 甲基球蛋白 (MetMb) 的形成通过暴露与脂质结合的疏水性区域来加速脂质氧化.
研究的目的:
- 为了研究梅拉德反应产物 (MRPs) 对鱼类肌肉中血红蛋白氧化的抑制作用.
- 评估MRPs对蛋白质氧化,质地和脂质氧化水平在洗的鱼-肌球蛋白模型中的影响.
- 评估MRP在保护鱼类质量的实际应用.
主要方法:
- 使用洗过的肌肉模型与鱼和肌球蛋白 (Mb).
- 评估的蛋白质氧化产物 (MetMb,碳基,基),蛋白质聚合,颗粒大小和脂质氧化.
- 在Mb介导的氧化模型中量化了初级和二级氧化代谢物的抑制.
主要成果:
- 在0.5%和1%度的MRPs抑制了Mb的氧化.
- 1%的MRPs使MetMb的形成减少了15.38%,蛋白质碳基因减少了6.53%,基减少了20.37%.
- MRPs显著降低了蛋白质聚合 (40.81%) 和颗粒大小 (36.95%),抑制了主要 (59.47%) 和次要 (68.19%) 的氧化代谢物,并保持了肌肉质地.
结论:
- MRPs作为有效的抗氧化剂,抑制鱼类肌肉中的肌红蛋白和脂质自氧化.
- 通过保持纹理和颜色,MRPs增强了普通鱼的稳定性.
- 马氏制剂为控制脂质氧化和提高冷藏鱼类产品质量提供了有价值的策略.
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