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一个染色体水平参考基因组的法师的圣人 (Salvia divinorum) 提供了洞察力萨尔维诺林A生物合成

Scott A Ford¹, Rob W Ness^2,3,4, Moonhyuk Kwon^5,6

¹Department of Cell and Systems Biology, University of Toronto, Toronto, ON, M5S 3G5, Canada.

BMC plant biology

|September 30, 2024

概括

研究人员对占卜师的圣人基因组进行了测序,以了解沙尔维诺林A (SalA) 生物合成. 这为探索SalA在疼痛和成治疗中的药用潜力提供了关键基因.

科学领域:

植物基因组学植物基因组学
自然产品的生物合成.
药理学药理学是指药理学的学科.

背景情况:

预言家的 (Salvia divinorum) 产生沙尔维诺林A (SalA),一种强大的K-阿片类受体激动剂.
在治疗慢性疼痛,成和PTSD方面,SalA具有潜在的治疗应用.
萨拉A的生物合成途径在很大程度上没有特征,以前只确定了两个步骤.

研究的目的:

为了生成高质量的,染色体水平的 Salvia divinorum 的基因组组合.
为了识别参与萨尔维诺林A生物合成的基因,A.
为了促进进一步研究SalA的药物应用.

主要方法:

染色体层次的基因组组装和注释.
鉴定二烯生物合成基因集群的鉴定.
参与SalA合成的基因家族的生物信息分析.
不同基因表达分析.

主要成果:

实现了541 Mb的双倍基因组组合,N50为41.4 Mb,BUSCO完整度为98.4%.
确定了两个关键的二二烯生物合成基因集群,包括克罗托诺利德G合成酶.
确定了下游SalA通路步骤的候选基因,但没有聚集.
大多数已识别的基因不能被甲基斯莫纳酸诱导.

结论:

高分辨率的基因组序列为Salvia属提供了宝贵的资源.
这些已识别的基因对于阐明完整的SalA生物合成途径至关重要.
这项工作使未来探索SalA的临床潜力成为可能.

关键词:

萨尔维亚神圣 (Salvia divinorum) 是一种植物.在新的基因组组装中.迪特尔类生物合成兰花科 (Lamiaceae) 是一个植物.药用植物药用植物新克洛二二二二二二二二二二二二二二二二二二二二二在KK阿片类受体.

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