相关实验视频

Updated: Jun 11, 2025

Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery

Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery

Published on: April 16, 2019

素通过调节O-GlcNAcylation来抑制肝细胞癌

Xiang Zhang¹, Li-Qiong Wang², Zhi-Yong Liu³

¹Department of Traditional Chinese Medicine, Qingpu Branch of Zhongshan Hospital Affiliated to Fudan University, Shanghai 201700, China.

World journal of gastrointestinal oncology

|October 1, 2024

概括

素减少O-GlcNAcylation和OGT表达,通过影响NF-κB和JNK通路来抑制肝细胞癌 (HCC) 的生长和转移.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
生物化学生物化学

背景情况:

肝细胞癌 (HCC) 的发病过程复杂,机制不清楚.
许多风险因素有助于HCC的发展.

研究的目的:

为了调查塞内基宁和O-GlcNAcylation在HCC生长和转移中的作用.
阐明在HCC中受到senegenin和O-GlcNAcylation影响的分子通路.

主要方法:

西部斑点分析检测HCC组织和细胞中的O-链接的N-乙糖胺转移酶 (OGT) 和O-GlcNAcylation水平.
在体外测试 (细胞计数套件-8,克隆原性,跨井迁移) 以评估原素和O-GlcNAcylation对HCC细胞增殖和转移的影响.
通过药物治疗和病毒感染操纵O-GlcNAcylation水平,然后对通路蛋白进行西部斑分析.

主要成果:

在HCC组织和细胞中,OGT和O-GlcNAcylation水平显著升高.
增加的O-GlcNAcylation与增强的增殖,侵入性,克隆性和转移潜力相关.
素治疗降低了O-GlcNAcylation,减缓了HCC细胞的增殖和迁移.
观察到核因子-kappa B (NF-κB) 和c-Jun N-终端激酶 (JNK) 途径中的蛋白质水平发生变化.

结论:

素有效降低了HCC中的O-GlcNAcylation和OGT表达.
素通过调节NF-κB和JNK通路来抑制HCC细胞生长和转移.
这项研究强调了塞内基宁作为一种潜在的治疗剂,针对HCC中的O-GlcNAcylation.

关键词:

肝癌是一种肝癌.转移转移转移转移这是一种O-GlcNAcylation.病变的发生和发病.参议员表示,我们已经做到了.

更多相关视频

Establishment of Hepatocarcinoma in BALB/c-nu Mice and Investigation of the Therapeutic Effect of the Sanleng Jiashen Formula

Establishment of Hepatocarcinoma in BALB/c-nu Mice and Investigation of the Therapeutic Effect of the Sanleng Jiashen Formula

Published on: January 26, 2024

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines

Published on: April 18, 2025

相关概念视频

Oligosaccharide Assembly

Oligosaccharide Assembly

Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...

Loss of Tumor Suppressor Gene Functions

Loss of Tumor Suppressor Gene Functions

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...

Cell Specific Gene Expression

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...

Liver Regeneration

Liver Regeneration

The liver is an important organ in vertebrates that plays an essential role in metabolism. It is also responsible for storing and redistributing nutrients such as carbohydrates, fats, and vitamins in the body. Additionally, the liver releases bile salts which are critical for digesting food and eliminating toxic metabolites from the body.
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are...

Proteoglycans

Proteoglycans

Glycans, a class of complex heterogeneous molecules, can be covalently attached to proteins to form glycosylated proteins that regulate various physiological and pathological processes. Glycosylated proteins or glycoproteins comprise N-linked and O-linked oligosaccharides. O-glycosylation is the most common type of protein glycosylation. Here, glycans attach to the oxygen atom of the hydroxyl groups of Serine or Threonine residues. O-linked glycosylation occurs later in protein processing,...