使用振动处理制备的生物药物能够在不与目标直接接触的情况下影响其目标
Elena Don1, Svetlana Zakharova1, Sabina Yaroshenko1
1R&D Department, OOO «NPF «Materia Medica Holding», Moscow, Russian Federation.
Dose-response : a publication of International Hormesis Society
|October 1, 2024
概括
用振动来处理药物溶液的技术处理 (TP),而不是物质含量,解释了它们的活动. 对胰岛素受体的TP抗体显著影响了细胞中的葡萄糖消耗,证明了物理效应.
科学领域:
- 药理学 药理学是指药理学的学科.
- 纳米技术纳米技术
- 细胞生物学 细胞生物学
背景情况:
- 对高度稀释的药物溶液的理解发生了转变,将活性归因于技术加工 (TP),而不是物质含量.
- 在稀释过程中,TP涉及振动,可能形成纳米粒子,并诱导原始物质中缺少的修饰活性.
- 通过在没有直接接触的情况下发生的效应表明TP溶液活动的物理性质.
研究的目的:
- 研究TP抗体对胰岛素受体对CHO细胞葡萄糖消耗的影响.
- 为了确定TP解决方案是否表现出与或没有直接接触暴露的活性.
- 阐明TP加工药物的活性背后的物理机制.
主要方法:
- 在连续稀释过程中使用振动制备TP溶液.
- 中国仓鼠卵巢 (CHO) 细胞暴露于针对胰岛素受体的TP抗体.
- 在接触和非接触暴露条件下测量细胞中的葡萄糖消耗.
- 统计分析比较TP治疗组与对照组 (P <0.05).
主要成果:
- 在大多数实验中观察到TP抗体对CHO细胞葡萄糖消耗的统计学显著影响 (P<0.05).
- 这种效应在接触和非接触暴露条件下都被证明,表明非局部活动.
- 对照组没有显示任何显著变化,突出显示TP抗体的特定影响.
结论:
- TP药物的活性与制备过程中的振动过程有关,而不是原始物质.
- 对胰岛素受体的TP抗体可以通过物理效应影响细胞葡萄糖代谢.
- 这些发现支持TP溶液活性的物理机制,在与细胞直接接触或不接触的情况下有效.
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