赛尔图因1/赛尔图因3是强大的氨酸脱甲基酶,而赛尔图因1介导的脱甲基酶调节糖解
Runhua Du1,2, Yanmei Gao3, Cong Yan2
1School of Pharmaceutical Science and Technology, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou 310024, China.
iScience
|October 1, 2024
概括
科学家们发现SIRT1和SIRT3酶消除了氨酸乳化 (Kla),这是将新陈代谢和基因调节联系在一起的标志. 这一发现揭示了超越表观遗传学的Kla的新调节器和功能.
科学领域:
- 生物化学 生物化学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 代谢调节 代谢调节 代谢调节 代谢调节
背景情况:
- 氨酸乳化 (Kla) 是一种由糖解和华堡效应等代谢途径调节的表观遗传修饰.
- 虽然像p300和HDAC1/3这样的酶是已知的调节剂,但Kla修饰酶及其基质的全谱仍然不完全理解.
研究的目的:
- 为了识别参与调节 lysine 乳糖化的新型酶.
- 阐明这些新发现的酶在细胞过程中的特定基质和功能作用.
主要方法:
- 对具有SIRT1和SIRT3基因淘汰的HepG2细胞进行了蛋白质组分析.
- 酶活性测定用于确认SIRT1和SIRT3在从蛋白质中去除乳基中的作用.
主要成果:
- 鉴定出SIRT1和SIRT3是消除 lysine乳酸化标记 (Kla) 的关键酶.
- 这些酶表现出明显的基质特异性,向素蛋白和非素蛋白.
- 证明Kla对酸盐激酶M2 (PKM2) 的修饰影响了代谢途径和细胞增殖.
结论:
- SIRT1和SIRT3充当了氨酸乳化的关键"清除器",扩大了这种表观遗传标记的已知的调节网络.
- 这项研究揭示了超越表观遗传学的氨酸乳化的新功能,影响细胞信号传递和代谢控制.
- 这些发现加深了对新陈代谢,表观遗传学和细胞功能之间的相互作用的理解.
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