SARS-CoV-2 费里丁纳米粒子疫苗产生具有广泛沙贝科病毒活动的超免疫马类血清
Elizabeth J Martinez1,2, William C Chang1,2, Wei-Hung Chen1,2
1Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
iScience
|October 1, 2024
概括
使用马匹SARS-CoV-2纳米粒子免疫原体的下一代疫苗迅速产生了保护性抗体. 马类血清中和了多种不同的萨尔贝科病毒,包括令人担忧的变体,提供了潜在的治疗益处.
科学领域:
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
- 疫苗学 疫苗学 疫苗学
背景情况:
- 新出现的SARS-CoV-2令人担忧的变种 (VoC) 和潜在的动物传染性溢出需要广泛的疫苗和治疗方法.
- 目前的策略需要不断更新,以应对不断变化的病毒威胁.
研究的目的:
- 评估SARS-CoV-2尖峰费里纳米粒子 (SpFN) 和受体结合域费里纳米粒子 (RFN) 免疫原体在马类模型中引起广泛的萨尔贝科病毒中和保护的能力.
- 评估马类衍生多克隆IgG对SARS-CoV-2VoC和其他沙贝科病毒的治疗潜力.
主要方法:
- 马匹用SARS-CoV-2 SpFN和RFN免疫原进行免疫.
- 超免疫血清和纯化的多克隆IgG被收集并测试了对各种SARS-CoV-2VoC和sarbecoviruses的中和和结合活性.
- 马类IgG的有效性在K18-hACE2转基因小鼠模型中进行了评估,该模型被挑战了Omicron XBB.1.5.5.
主要成果:
- 免疫迅速诱导的血清具有强大的中和SARS-CoV-2 VoC和SARS-CoV-1伪病毒.
- 塞拉显示强大的结合受体结合域从多个sarbecovirus类型 (1b,1a,2,3和4).
- 在小鼠模型中,纯化马体IgG在小鼠模型中提供了对Omicron XBB.1.5的保护.
结论:
- 马类的SARS-CoV-2纳米粒子疫苗可以快速产生针对sarbecoviruses的广泛和保护性免疫反应.
- 马类血清IgG显示出对新出现的SARS-CoV-2VoC和各种sarbecoviruses的治疗承诺,可能补充单克隆抗体疗法.
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