通过转录组学识别新的生物标志物,共享的分子签名和免疫细胞透在心脏和功能衰竭的转录组学
Qingqing Long1, Xinlong Zhang2, Fangyuan Ren3
1Division of Nephrology and Clinical Immunology, Medical Faculty, Rheinisch-Westfälische Technische Hochschule (RWTH) Aachen University, Aachen, Germany.
Frontiers in immunology
|October 1, 2024
概括
这项研究确定了CDK2和CCND1作为关键生物标志物,将细胞周期和心脏和功能衰竭中的炎症联系起来,为这些常见疾病提供了新的诊断和治疗标.
科学领域:
- 生物医学研究的研究.
- 分子生物学分子生物学
- 心血管和脏医学.
背景情况:
- 心力衰竭 (HF) 和功能衰竭 (KF) 经常同时存在,这带来了重大的临床挑战.
- 在HF和KF之间共享的分子机制尚未完全理解,这阻碍了有效的治疗开发.
研究的目的:
- 为了确定同时发生的HF和KF的新型分子特征和生物标志物.
- 阐明HF和KF中常见的潜在生物途径和免疫反应.
主要方法:
- 转录组分析以确定在HF和KF患者中共享的差异表达基因 (DEGs).
- 构建一个蛋白质-蛋白质相互作用 (PPI) 网络,并应用机器学习算法 (RF,SVM-RFE,LASSO) 来识别特征基因.
- 功能丰富分析 (GSVA,GSEA),分子对接和免疫细胞透分析.
主要成果:
- 鉴定了2869个共享的DEGs,在细胞平衡,炎症和细胞外矩阵生产中具有丰富性,特别是PI3K-Akt信号通路.
- 确定了CDK2和CCND1作为关键的签名基因,验证了诊断价值,并与细胞循环和炎症有关.
- 分子对接和免疫分析揭示了CDK2和CCND1与HF和KF的免疫微环境的关联.
结论:
- CDK2和CCND1是连接细胞循环调节和HF和KF炎症的新生物标志物.
- 这些发现为HF和KF的分子病原发生提供了新的见解.
- 已识别的生物标志物为未来的诊断和治疗策略提供了潜在的目标.
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