新的细菌阿里硫转移酶:有效的工具硫化聚醇
Katerina Brodsky1,2, Barbora Petránková1,3, Lucie Petrásková1
1Institute of Microbiology of the Czech Academy of Sciences, Vídeňská 1083, Prague 4 CZ-142 00, Czech Republic.
Journal of agricultural and food chemistry
|October 1, 2024
概括
纯生物活性化合物代谢物,如多,对于药理学至关重要. 这项研究使用新型细菌硫转移酶 (AST) 酶化合成了这些代谢物,使得有效生产基本标准成为可能.
科学领域:
- 生物化学 生物化学
- 酶学 是一种酶学.
- 药理学 药理学是指药理学的学科.
背景情况:
- 准备纯生物活性化合物代谢物,特别是多,对于体内药理学研究至关重要.
- 使用细菌PAPS独立的硫转移酶 (AST) 的酶合成为繁的提取方法提供了替代方案.
- 有限的特征性AST已经可用于聚硫化.
研究的目的:
- 引入和描述用于酶性多硫化新型重组硫转移酶 (AST) 的特性.
- 为了选这些AST,以检查它们硫化各种类黄和酸的能力.
- 通过产生和表征新的代谢物标准来证明这种酶方法的实用性.
主要方法:
- 在大肠杆菌 (Escherichia coli) 中选择和产生复合性AST.
- 精选的ASTs的净化和生物化学表征.
- 用p-nitrophenyl硫酸盐作为基质对9种黄和2种酸硫化AST进行选.
主要成果:
- 所有测试的化合物都是新型AST的基质.
- 凯姆菲罗尔和黄素是硫化化合物中效率最高的化合物.
- 来自Desulfofalx alkaliphile (DalAST) 和Campylobacter胎儿 (CfAST) 的AST表现出最高的硫化效率.
- 新的真实代谢物标准,特别是二硫酸的区域异构体,已成功合成和表征.
结论:
- 新型细菌AST有效硫化一系列多,包括黄和酸.
- 对于多硫化,DalAST和CfAST特别有效.
- 这种酶合成方法提供了一种可靠的方法来产生纯的多代谢物和药理学研究的标准.
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