在 holo-TFIID 枯竭的小鼠胚胎干细胞中RNA聚合酶II转录的启动
Vincent Hisler1, Paul Bardot1, Dylane Detilleux1
1Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), 67400 Illkirch, France; CNRS, UMR7104, 67400 Illkirch, France; INSERM, U1258, 67400 Illkirch, France; Université de Strasbourg, 67400 Illkirch, France.
Cell reports
|October 1, 2024
概括
五分之一的钱.
科学领域:
- 分子生物学分子生物学
- 基因规则 基因规则
- 生物化学 生物化学
背景情况:
- TFIID通过识别核心促进子序列来启动RNA聚合酶II (Pol II) 转录.
- 甲动物全体TFIID是TATA结合蛋白 (TBP) 和13个TBP相关因子 (TAF) 的综合体.
- 在TFIID复合体形成和转录启动中,TAFs的确切作用尚未完全理解.
研究的目的:
- 调查TAF7和TAF10对于TFIID复合体形成和功能的必要性.
- 阐明TAFs对转录启动和Pol II调节的贡献.
- 了解部分TFIID复合组合对细胞过程的影响.
主要方法:
- 在小鼠胚胎干细胞中,TAF7或TAF10的诱导性衰竭.
- 对TFIID亚复合体形成,染色体结合和新生转录的分析.
- 综合评估TFIID复合物的稳定性和随时间推移的功能.
主要成果:
- TAF7的耗尽导致TFIID缺乏TAF7,而TAF10的耗尽产生了最小的核心-TFIID.
- 两个部分复合体最初都支持TBP招募和新生的Pol II转录.
- 发现TAF10对于高效的Pol II暂停至关重要.
- 部分组装的TFIID复合体在发育过程中或在多个细胞分裂过程中无法完全取代全体TFIID.
结论:
- TAFs,特别是TAF10,在保持全体TFIID的完整性和功能方面发挥着至关重要的作用.
- 虽然部分TFIID复合体可以启动转录,但它们对于持续的细胞过程和发育是不够的.
- 这项研究强调了全体TFIID结构对于强大的基因表达调节的重要性.
相关概念视频
01:47Transcription Initiation
16.3K
Initiation is the first step of transcription in eukaryotes. Prokaryotic RNA Polymerase (RNAP) can bind to the template DNA and start transcribing. On the other hand, transcription in eukaryotes requires additional proteins, called transcription factors, to first bind to the promoter region in the DNA template. This binding helps recruit the specific RNAP that can assemble on the DNA and start transcription.
The promoters and enhancers and their accessory proteins allow tight regulation of...
The promoters and enhancers and their accessory proteins allow tight regulation of...
16.3K
01:30General Transcription Factors
5.2K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
5.2K
02:36RNA Polymerase II Accessory Proteins
9.1K
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
9.1K
01:53Bacterial Transcription
28.0K
RNA polymerase (RNAP) carries out DNA-dependent RNA synthesis in both bacteria and eukaryotes. Bacteria do not have a membrane-bound nucleus. So, transcription and translation occur simultaneously, on the same DNA template.
Transcription can be divided into three main stages, each involving distinct DNA sequences to guide the polymerase. These are:
Transcription can be divided into three main stages, each involving distinct DNA sequences to guide the polymerase. These are:
28.0K
02:35Transcription Elongation Factors
10.8K
Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene.
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA...
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA...
10.8K
00:58Eukaryotic RNA Polymerases
24.0K
RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
All three eukaryotic RNAPs require specific transcription factors, of which the...
24.0K