象牙 lncRNA 调节眼蝶的季节性颜色模式
Richard A Fandino1, Noah K Brady1, Martik Chatterjee1
1Department of Ecology and Evolutionary Biology, Cornell University, Ithaca, NY 14853.
概括
一种保存的长非编码RNA (lncRNA),象牙,是蝶颜色模式的关键基因. 象牙控制季节性颜色变化和Junonia coenia的其他翅膀色素变化.
科学领域:
- 进化发育生物学 进化发育生物学
- 基因组学就是基因组学.
- 分子生物学分子生物学
背景情况:
- 长非编码RNAs (lncRNAs) 调节基因表达,但它们的进化作用尚不清楚.
- 类动物的皮质位置与密码和模仿有关,但涉及的特定基因仍在争论中.
- 了解IncRNA在适应中的功能对于进化研究至关重要.
研究的目的:
- 确定负责Junonia coenia.在皮质位点的颜色模式的特定基因.
- 研究lncRNA象牙在季节性色彩图案可塑性和翅膀色素化中的作用.
- 阐明控制象牙在发育过程中的表达的调节机制.
主要方法:
- 遗传关联研究将象牙内部的cis-regulatory元素 (CREs) 与颜色模式的自然变异联系起来.
- 对蝶进行象牙CREs有针对性的删除的表型分析.
- 使用ChIP试验对象牙促进体结合的转录因子的分析.
主要成果:
- lncRNA象牙,而不是皮质,被确定为这个位置的主要颜色模式基因.
- 象牙第一个内部的特定CREs与季节性色彩可塑性有遗传关联.
- 象牙CREs的删除会导致翅膀颜色变化,包括眼球环和整体颜色强度的变化.
- 关键的转录因子 (Spineless,Bric-a-brac,Ftz-f1) 与象牙促进体结合,表明直接调节.
结论:
- lncRNA象牙在蝶颜色模式的进化和发展中起着重要作用.
- 单个lncRNA的Cis调节可以产生多样化和细微的表型效应,包括季节性可塑性.
- 这项研究强调了 lncRNAs 作为进化适应的目标的重要性.
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