揭示了SARS-CoV-2进入宿主细胞的动态机制,在单个粒子水平
Siying Li1, Hui Yang2,3, Falin Tian4
1School of Chemistry and Life Science, Advanced Institute of Materials Science, Changchun University of Technology, Changchun 130012, China.
ACS nano
|October 1, 2024
概括
SARS-CoV-2 的 Omicron 变种比 Delta 或野生型更强烈地与 ACE2 受体结合. 奥米克龙还通过克拉依赖性内细胞分裂更快,更有效地进入细胞.
科学领域:
- 病毒学 病毒学
- 生物物理学的生物物理.
- 细胞生物学 细胞生物学
背景情况:
- 了解严重急性呼吸道冠状病毒2 (SARS-CoV-2) 细胞进入是COVID-19药物开发的关键.
- 病毒结合动态和病原体需要详细的研究.
研究的目的:
- 研究SARS-CoV-2结合细胞膜和进入机制的动态特征.
- 为了比较不同SARS-CoV-2变体的结合亲和和和进入动态,包括Omicron.
主要方法:
- 基于原子力显微镜 (AFM) 的单分子力光谱 (SMFS) 用于研究病毒-细胞膜相互作用.
- 使用实时力追踪和单粒子光追踪.
- 粗粒度分子动力学 (CGMD) 模拟用于参数分析.
主要成果:
- 奥米克朗变异病毒样颗粒 (VLPs) 对ACE2受体的结合亲和力比三角形和野生型 (WT) 强.
- SARS-CoV-2 VLP 进入细胞需要大约 200 ms 和 60 pN,与 CGMD 模拟保持一致.
- 奥米克朗变种表现出更高的细胞进入速度,更低的力,以及更高的概率,利用克拉素依赖性内细胞分裂.
结论:
- 该研究提供了对SARS-CoV-2 VLP入口的动态参数的定量见解.
- 奥米克龙变种表现出明显的,更高效的细胞入侵特征.
- 这些发现提供了针对SARS-CoV-2进入机制的潜在干预策略.
更多相关视频
08:40Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting
Published on: March 1, 2019
58.9K
06:39High-throughput Confocal Imaging of Quantum Dot-Conjugated SARS-CoV-2 Spike Trimers to Track Binding and Endocytosis in HEK293T Cells
Published on: April 21, 2022
3.0K
相关概念视频
01:10Intracellular Movement of Viruses and Bacteria
2.8K
Intracellular bacteria and viruses often comprise a group of highly infectious pathogens that can cause several diseases. Bacterial pathogens include those belonging to the genus Rickettsia responsible for conditions such as rocky mountain spotted fever and the Mediterranean spotted fever; Chlamydia, a genus responsible for a sexually transmitted disease; Coxiella burnetii, an agent responsible for Q fever. Viral pathogens include vaccinia—a poxvirus, and herpes simplex virus—a...
2.8K
02:50Conjugated Proteins
18.2K
Simple proteins and protein complexes contain only amino acids. In contrast, many other proteins, called conjugated proteins, covalently bond with non-protein moieties.
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
18.2K
00:56Viral Structure
61.9K
Viruses are extraordinarily diverse in shape and size, but they all have several structural features in common. All viruses have a core that contains a DNA- or RNA-based genome. The core is surrounded by a protective coat of proteins called the capsid. The capsid is composed of subunits called capsomeres. The capsid and genome-containing core are together known as the nucleocapsid.
61.9K
01:20Receptor-mediated Endocytosis
6.0K
Receptor-mediated endocytosis is when bulk amounts of specific molecules are imported into a cell after binding to cell surface receptors. The molecules bound to these receptors are taken into the cell through inward folding of the cell surface membrane, which is eventually pinched off into a vesicle within the cell. Structural proteins, such as clathrin, coat the budding vesicle.
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...
6.0K