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在斑马鱼中抑制食物摄入的一种神经的鉴定

Fengying Yan¹, Zhiqiang Hao¹, Junyan Zeng¹

¹State Key Laboratory of Biocontrol and School of Life Sciences, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), and Guangdong Provincial Key Laboratory for Aquatic Economic Animals, Sun Yat-Sen University, Guangzhou, 510275, China.

Biochemical and biophysical research communications

|October 1, 2024

概括

一种新发现的神经,Nwk,调节斑马鱼的食欲. 这项研究表明,Nwk通过影响关键的食欲调节基因来减少食物摄入量,突出其在养行为中的作用.

科学领域:

神经科学是一个神经科学.
遗传学是一个遗传学.
内分泌学在内分泌学.

背景情况:

神经是脊椎动物生理功能的重要调节者.
了解新的神经信号通路对于破译复杂的生物过程至关重要.

研究的目的:

在脊椎动物中识别和表征一种新的神经基因,nwk.
研究Nwk神经在调节斑马鱼的食物摄入量和相关基因表达中的作用.

主要方法:

基因鉴定和cDNA克隆斑马鱼大脑中的nwk.
对Nwk前体和成熟序列的分析.
在特定的大脑区域进行定量mRNA表达分析.
使用内注射Nwk-22.2.的功能研究.
使用CRISPR/Cas9技术生成和分析nwk淘汰赛斑马鱼.

主要成果:

一个新的神经前体 (Nwk) 和成熟的 (Nwk-22) 被识别和特征.
斑马鱼大脑的Nwk表达局限于TPp和前视区域.
Nwk-22的使用抑制了食物摄入,并降低了agrp mRNA的表达.
现在的淘汰赛斑马鱼表现出增加的食物消费.
在淘汰鱼类中,Nwk-22治疗恢复了抑制的agrp表达和调高的pomca表达.

结论:

在斑马鱼中,Nwk 作为一种厌食性神经的功能.
Nwk通过降低氧化性agrp的调节和上调无氧化性pomc基因表达来减少食物摄入量.
这项研究阐明了一种参与中央调节食欲的新型神经路径.

关键词:

食物摄入量食物摄入量在 Nwk Nwk 斑马鱼是一种斑马鱼.

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