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脱氧尼瓦伦醇暴露会扰乱猪卵细胞中细胞质成熟的过程

Lin-Lin Hu¹, Ya-Xi Liu², Xiao-Ting Yu³

¹Key Laboratory of Research on Clinical Molecular Diagnosis for High Incidence Diseases in Western Guangxi of Guangxi Higher Education Institutions, Reproductive Medicine of Guangxi Medical and Health Key Discipline Construction Project, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise 533000, China; Industrial College of Biomedicine and Health Industry, Youjiang Medical University for Nationalities, Baise 533000, China.

Ecotoxicology and environmental safety

|October 1, 2024

概括

脱氧尼瓦伦醇 (DON) 毒素在成熟过程中破坏了关键的猪卵细胞器官,包括线粒体和内分泌网膜. 这会影响细胞功能,影响生育和生殖.

科学领域:

生殖生物学生殖生物学
毒理学毒理学毒理学
细胞生物学细胞生物学

背景情况:

脱氧尼瓦伦醇 (DON) 是一种Fusarium真菌毒素,在食品中普遍存在.
DON具有显著的毒性,影响人类和动物的生长,发育和繁殖.
猪卵细胞在成熟期间对DON对猪卵细胞器官的影响尚不清楚.

研究的目的:

调查DON对猪卵细胞器官的有毒作用,特别是线粒体,内分泌网膜和戈尔吉装置.
阐明DON对猪卵细胞成熟过程中的关键过程的影响,包括细胞间通信和介质进展.

主要方法:

猪卵细胞在体外成熟过程中暴露于DON.
用光显微镜评估器官功能,以评估线粒体膜潜力,溶酶体完整性和内分泌网膜压力标志物 (GRP78).
分析了自标志物 (LC3) 和戈尔吉装置分布 (GM130).

主要成果:

DON暴露抑制了颗粒细胞扩散和第一个极体挤出.
线粒体功能 (光强度,膜潜力) 和 lysosomal 完整性显著改变.
DON诱导了内等质网膜应激 (高调节的GRP78) 和破坏了戈尔吉器官的分布和表达 (GM130).
增加的LC3光表明增强了自,表明蛋白质降解.

结论:

暴露于DON会对关键的猪卵细胞器官产生不利影响,包括线粒体,内质网膜和戈尔吉装置.
这些干扰会在卵细胞半转化和成熟过程中损害细胞的基本功能,这可能会影响生殖结果.
这些发现强调了DON作为对猪卵细胞质量和生育能力的重大威胁.

关键词:

脱氧尼瓦伦 (deoxynivalenol) 是一种除氧化.介质变化 (Meiosis) 是一个过程.菌毒毒素是一种毒素.卵子细胞是卵子细胞.有机器人有机器人

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