肠道和呼吸道微生物群的改变对臭氧诱导的呼吸道炎症的影响
1School of Public Health, Xinxiang Medical University, Xinxiang, Henan Province 453003, China.
The Science of the total environment
|October 1, 2024
概括
地平面臭氧 (O3) 暴露会导致呼吸道炎症,破坏肠道和呼吸道微生物并改变新陈代谢. 抗生素治疗加剧了这些影响,将微生物群和代谢变化与臭氧诱导的炎症联系起来.
科学领域:
- 环境健康 环境健康
- 微生物学 微生物学
- 毒理学 毒理学 毒理学
背景情况:
- 地平面臭氧 (O3) 是一种已知的呼吸道刺激剂.
- 在O3诱导的气道炎症背后的精确机制尚未完全理解.
- 肠道和呼吸道微生物群的作用以及O3毒性的代谢变化需要进一步研究.
研究的目的:
- 调查肠道和呼吸道微生物群失调,呼吸道代谢变化和O3诱导的呼吸道炎症之间的关联.
- 确定广谱抗生素对O3诱导的呼吸道炎症以及相关的微生物和代谢变化的影响.
主要方法:
- C57BL/6N小鼠被暴露在过空气或1ppm O3中5天,先前接受或没有接受广泛抗生素治疗.
- 便和支气管洗液 (BALF) 样本进行了氧化应激,炎症生物标志物,微生物群组成 (16S rRNA测序) 和代谢物概况分析.
- 肺部组织被用血素-色素染色方法对其进行了本病理学检查.
主要成果:
- 暴露于O3引发了呼吸道氧化应激,炎症,肠道和呼吸道微生物群失调,以及代谢变化.
- 抗生素预治疗加剧了O3诱导的代谢障碍和呼吸道炎症.
- 斯皮尔曼相关性分析揭示了肠道/呼吸道微生物群失调,代谢障碍和O3诱导的呼吸道炎症之间的关联.
结论:
- 吸入的O3会引发气道炎症.
- 肠道和呼吸道微生物群失调和呼吸道代谢变化与O3诱导的呼吸道炎症的病原发生有关.
- 微生物群的调节可能会影响O3诱导的呼吸效应的严重程度.
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