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相关概念视频

Replication in Prokaryotes01:32

Replication in Prokaryotes

24.8K
DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
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S-Cdk Initiates DNA Replication02:38

S-Cdk Initiates DNA Replication

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The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of...
4.7K
Chromosome Replication02:31

Chromosome Replication

8.7K
Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins.  This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin...
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Replication in Eukaryotes02:31

Replication in Eukaryotes

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Overview
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Lagging Strand Synthesis01:59

Lagging Strand Synthesis

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During replication, the complementary strands in double-stranded DNA are synthesized at different rates. Replication first begins on the leading strand. Replication starts later, occurs more slowly, and proceeds discontinuously on the lagging strand.
There are several major differences between synthesis of the leading strand and synthesis of the lagging strand. 1) Leading strand synthesis happens in the direction of replication fork opening, whereas lagging strand synthesis happens in the...
50.3K
DNA Replication02:40

DNA Replication

49.0K
DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied.  After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
DNA replication...
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相关实验视频

Updated: Jun 13, 2025

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
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从单细胞DNA测序数据推断复制时间和增殖动态.

Adam C Weiner1,2, Marc J Williams1, Hongyu Shi1,3

  • 1Computational Oncology, Department of Epidemiology and Biostatistics, Memorial Sloan Kettering Cancer Center, New York, NY, USA.

Nature communications
|October 1, 2024
PubMed
概括

我们开发了一种新的计算方法来研究癌细胞中的DNA复制和拷贝数变化. 这种方法有助于了解基因组不稳定性如何驱动形瘤中的癌症进化.

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Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement
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Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization

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科学领域:

  • 基因组学就是基因组学.
  • 癌症生物学 癌症生物学
  • 计算生物学 计算生物学

背景情况:

  • 在癌症中常见的形积分症涉及DNA复制和细胞分裂中的错误.
  • 副本数变化 (CNAs),复制时间 (RT) 和无体瘤细胞周期动态之间的关系尚不清楚.

研究的目的:

  • 从单细胞全基因组测序 (scWGS) 数据中推断细胞特异复制和复制数状态的计算方法.
  • 在各种状癌症模型中研究克隆特异性RT和扩散动态.

主要方法:

  • 开发了PERT,一种用于同时推断细胞特异复制和副本数量状态的概率方法.
  • 应用PERT分析了来自细胞系,异种移植和原发性癌症的5万多个细胞.

主要成果:

  • 观察到RT和CNA之间的双向关系,X-无活化CNA导致了显著的RT转移.
  • 发现S相丰富与稳定的细胞中的增殖率相关,但不稳定的细胞中没有.
  • 使用scWGS数据证明了S相细胞的强大计算识别.

结论:

  • 复制时间和细胞周期属性对于了解形瘤的基因组进化至关重要.
  • PERT为分析scWGS数据提供了一个强大的工具,用于研究癌细胞动态.