在珍珠小米中使用全基因组SNP和SV进行农学表型的基因组选择
Haidong Yan1,2, Yarong Jin1, Haipeng Yu3
1College of Grassland Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
概括
结构变异 (SV) 标记在珍珠小米育种中比单核酸多态 (SNP) 标记提供更高的遗传多样性和预测准确性. 这项研究确定了使用这些先进的分子标记物进行作物改进的有希望的候选加入.
科学领域:
- 植物遗传学和基因组学
- 提高作物质量和培育作物质量
- 生物信息学和统计遗传学
背景情况:
- 珍珠小米是一种重要的,抗压作物,但由于分子辅助育种的有限使用,其繁殖进展受到阻碍.
- 现有的育种策略往往未充分利用了生殖质中可用的全谱遗传变异.
研究的目的:
- 评估单核酸多态 (SNP) 和结构变异 (SV) 标记在珍珠小米基因组预测中的实用性.
- 为了比较SNP和SV标记器在捕捉遗传多样性和预测表型方面的表现.
- 为了确定精英珍珠小米加入加速繁殖计划.
主要方法:
- 从242个珍珠小米杂交加入中收集了1,455,924个SNP和124,532个SV标记.
- 评估了120种与产量相关的表现型.
- 评估了九个基因组预测模型,比较了SNP和SV标记器的性能.
主要成果:
- SV标志物揭示了SNP标志物无法捕捉到的遗传多样性.
- 与SNP标志物相比,SV标志物在各种模型中表现出更高的遗传性和预测准确性.
- 基因组最佳线性无偏预测 (GBLUP) 使用 SV 标记和使用 SNP 标记的贝叶斯方法分别显示出最佳性能.
- 使用SNP识别了8个候选加入,并使用高基因组估计繁殖值 (GEBV) 的SV标记器识别了4个候选加入.
结论:
- SV标记物为SNP标记物提供了有价值的补充物,用于对珍珠小米进行全面的基因组预测.
- 该研究确定了特定的精英加入,包括"P23"的恐慌号,加速分子育种努力.
- 结果支持将SNP和SV标记数据整合起来,以加强珍珠小米品种的发展.
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