Circ_0015382 调节miR-942-5p/NDRG1轴以抑制热囊细胞功能
1Obstetrical Department, Northwest Women's and Children's Hospital, No.1616 Yanxiang Road, Qujiang New District, Xi'an, Shaanxi, 710061, China.
Reproductive sciences (Thousand Oaks, Calif.)
|October 1, 2024
概括
循环RNA (circ_0015382) 通过调节miR-942-5p/NDRG1通路来抑制孕前的进展. 这项研究揭示了一种新的分子机制,它是孕前发展的基础.
科学领域:
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
- 人体生理学 人体生理学
背景情况:
- 循环RNAs (circRNAs) 在人类疾病中至关重要,包括先兆子 (PE).
- Circ_0015382与PE进展有关,需要进一步的机制研究.
- 了解circRNA的作用对于开发PE新型治疗策略至关重要.
研究的目的:
- 为了阐明circ_0015382在产前 (PE) 中的分子机制.
- 调查PE中circ_0015382,miR-942-5p和NDRG1之间的监管关系.
- 为了确定circ_0015382对热囊细胞功能的影响.
主要方法:
- 定量PCR和西方布洛特用于基因和蛋白质表达分析.
- 细胞增殖,侵袭和血管生成的MTT,EdU,transwell和管道形成试验.
- 双 luciferase 记者和RNA 免疫沉降测定以确认分子相互作用.
主要成果:
- 在PE胎盘组织中,Circ_0015382和NDRG1的调节上升,而miR-942-5p的调节下降.
- Circ_0015382 敲击促进了 trofhoblast 细胞的增殖,入侵和血管生成.
- Circ_0015382被为miR-942-5p,而NDRG1是miR-942-5p的一个目标.
结论:
- Circ_0015382 作为对PE相关的热囊细胞功能的抑制剂.
- 该circ_0015382/miR-942-5p/NDRG1轴为PE提供了一个新的治疗目标.
- 这项研究为孕前的分子病变产生提供了新的见解.
相关概念视频
01:22MicroRNAs
3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
02:23Master Transcription Regulators
6.9K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.9K
02:19Circadian Rhythms and Gene Regulation
4.0K
The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent...
4.0K
01:16TGF - β Signaling Pathway
7.3K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.3K
01:24Regulation of Angiogenesis and Blood Supply
2.5K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.5K
02:39lncRNA - Long Non-coding RNAs
8.5K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.5K