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模块化,可扩展和可定制的LC-HRMS用于Exposomics

Vinicius Verri Hernandes1,2, Benedikt Warth3,4

  • 1Department of Food Chemistry and Toxicology, Faculty of Chemistry, University of Vienna, Vienna, Austria.

Methods in molecular biology (Clifton, N.J.)
|October 1, 2024
PubMed
概括

这项研究提出了一种多功能液体染色学高分辨率质谱法 (LC-HRMS) 方法,用于非向性曝光学分析. 该工作流程通过使用数据依赖的获取和包含列表在人体样本中增强有毒物质的识别.

关键词:
分析毒理学分析毒理学生物监测 生物监测数据依赖分析数据依赖分析环境和食品污染物的污染物.暴露性研究是暴露性的研究.高分辨率质谱测量高分辨率质谱测量没有针对性的代谢组和暴露组.

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科学领域:

  • 环境化学环境化学
  • 分析化学 分析化学
  • 毒理学 毒理学 毒理学

背景情况:

  • 暴露学研究旨在全面测量生物样本中的所有外源性和内源性小分子.
  • 准确和敏感的分析方法对于广泛的异生菌的非向检测至关重要.
  • 现有的工作流程可能需要优化,以便在人类矩阵中进行广泛的毒性物质分析.

研究的目的:

  • 描述一个灵活的反相液态色谱-高分辨率质谱 (LC-HRMS) 工作流程,用于非目标的曝光学.
  • 通过数据依赖获取 (DDA) 和包含列表来实现对毒性物质的半向分析.
  • 为了在人体血和血清中提供超过160种不同的异生菌的预期保留时间.

主要方法:

  • 开发一个多用途的LC-HRMS方法.
  • 实施数据依赖采集 (DDA) 进行数据采集.
  • 使用有毒物质含有清单,以加强对异生菌的识别.
  • 对人体血和血清样本的分析.

主要成果:

  • 建立了一个强大的LC-HRMS工作流程,以获得高质量的曝光学数据.
  • 产生了>160种异生菌的预期保留时间,有助于化合物识别.
  • 证明了DDA与含有列表相结合的实用性,用于半向性毒素分析.

结论:

  • 描述的LC-HRMS工作流作为一个有价值的工具用于exposomics研究和教育.
  • 该方法可以适应专门的研究需求,包括与数据独立获取 (DIA) 集成.
  • 这种方法促进了全面的毒性物质分析和环境暴露的理解.