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相关概念视频

Mass Spectrometry: Isotope Effect01:13

Mass Spectrometry: Isotope Effect

2.0K
Most elements exist in nature as a mixture of isotopes. The isotopes differ in weight due to their respective number of neutrons. The molecular weight of a molecule is different depending on the specific isotope of its elements involved. As a result, the mass spectrum of the molecule exhibits peaks from the same fragment at multiple positions. The positions of these mass signals depend on the difference between the molecular mass. Furthermore, the intensity of these signals is dependent on the...
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Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
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相关实验视频

Updated: Jun 11, 2025

Author Spotlight: Quantification of Complex Lipidomic Samples Using Stable Isotope Labeling
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使用LC-MS进行稳定同位素追踪实验.

Esther A Zaal1,2, Jeroen W A Jansen3,4, Celia R Berkers3,4

  • 1Division of Cell Biology, Metabolism & Cancer, Department of Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, the Netherlands. e.a.zaal@uu.nl.

Methods in molecular biology (Clifton, N.J.)
|October 1, 2024
PubMed
概括
此摘要是机器生成的。

代谢学研究使用同位素追踪来动态地绘制细胞代谢,这对于理解癌症等疾病和开发新疗法至关重要. 这种方法揭示了新陈代谢的重新连接,以改善诊断和药物反应.

关键词:
流动学 (Fluxomics) 是一种流动学.高性能液体色谱学 高性能液体色谱学同位素追踪是一种同位素追踪.质谱测量质量谱测量代谢学 代谢学 代谢学

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科学领域:

  • 生物化学和分子生物学
  • 系统生物学 系统生物学
  • 代谢学 代谢学 代谢学

背景情况:

  • 代谢学对于理解细胞功能和疾病机制至关重要.
  • 代谢变化是癌症等疾病的关键驱动因素,影响疾病的进展和治疗结果.
  • 识别代谢重新连接对于开发新的诊断和治疗策略至关重要.

研究的目的:

  • 概述一个强大的同位素追踪协议,用于分析细胞代谢.
  • 为研究人员在细胞培养模型中使用同位素追踪提供一个有价值的资源.
  • 详细说明高分辨率质谱学与液态色谱学相结合的应用,用于代谢研究.

主要方法:

  • 利用同位素追踪通过细胞生化反应动态追踪标记的基质.
  • 采用高分辨率质谱与液体色谱相结合,用于敏感的检测.
  • 在基于细胞培养的模型中应用该协议进行全面分析.

主要成果:

  • 该研究提供了对同位素追踪实验的详细协议.
  • 它涵盖了实验设计和数据分析的基本方面.
  • 描述的方法使我们能够动态地了解细胞代谢途径.

结论:

  • 同位素追踪是一种强大的技术,用于阐明细胞代谢线路.
  • 概述的协议有助于研究在疾病背景下代谢重新连接.
  • 这种方法支持在代谢学中发现新的诊断和治疗策略.