逆相超高性能液体染色学-质谱法用于高通量脂质量量定量
Zuzana Vaňková1, Ondřej Peterka1, Robert Jirásko1
1Faculty of Chemical Technology, Department of Analytical Chemistry, University of Pardubice, Pardubice, Czech Republic.
Methods in molecular biology (Clifton, N.J.)
|October 1, 2024
概括
一种新的逆相超高性能液态染色体质谱法 (RP-UHPLC/MS) 方法准确量化了人体血和血清中的众多脂类物种. 这种有针对性的脂质组分析增强了用于生物研究的脂质分析.
科学领域:
- 分析化学 分析化学
- 生物化学 生物化学
- 质谱测量质量谱测量
背景情况:
- 脂管学对于理解生物过程和疾病至关重要.
- 在等离子体和血清等生物矩阵中精确量化各种脂类物种,这给分析带来了挑战.
- 现有的方法可能缺乏全面的脂质分析的分辨率或吞吐量.
研究的目的:
- 开发和优化一个强大的逆相超高性能液体染色体质谱法 (RP-UHPLC/MS) 方法.
- 为了能够准确量化23个子类中的大量脂类物种.
- 为了方便在人体血和血清样本中进行详细的脂质组分析.
主要方法:
- 使用C18桥接乙烯混合 (BEH) 列进行脂质分离.
- 在正离子模式下使用三重四极质谱仪与多重反应监测 (MRM).
- 实施了用于数据评估的Skyline软件和用于精确量化的内部标准.
主要成果:
- 在25分钟的运行时间内,从23个子类中实现了脂质的分离.
- 证明了异构和异构脂质形式的分辨率,提高了分析特异性.
- 建立了人类血和血清中脂类物种的定量方法.
结论:
- 优化的RP-UHPLC/MS方法为有针对性的脂质组分析提供了强大的工具.
- 这种技术允许对广泛的脂质进行高通量和精确的定量测量.
- 该方法适用于临床和研究环境中的全面脂质分析.
相关概念视频
01:11High-Performance Liquid Chromatography: Introduction
1.6K
High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
In HPLC, two phases play a critical role in the separation process:
1.6K
01:05High-Performance Liquid Chromatography: Elution Process
435
In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
435