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高通量RPLC-MS/MS对短链和中链脂肪酸的量化

Stefan Schauer1, Alaa Othman2

  • 1Functional Genomics Center Zurich, ETH Zurich and University of Zurich, Zurich, Switzerland.

Methods in molecular biology (Clifton, N.J.)
|October 1, 2024
PubMed
概括

这项研究提出了一种快速而灵敏的方法,用于量化人体生物流体中短链和中链脂肪酸 (SMCFA) 和乳酸. 新技术为代谢研究提供了更好的吞吐量和精度.

关键词:
3 - - 尼托基氨酸的衍生化.分支短链脂肪酸 分支短链脂肪酸癌症治疗治疗 癌症治疗我们的肠道微生物组.免疫系统的免疫系统.中链脂肪酸 中链脂肪酸个性化医疗是个性化的医疗.短链脂肪酸 短链脂肪酸信号分子的信号分子.有针对性的代谢组学.

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Mass Spectrometry: Long-Chain Alkane Fragmentation01:18

Mass Spectrometry: Long-Chain Alkane Fragmentation

The molecular ions of linear alkanes prefer to fragment at the carbon-carbon bond away from the end of the chain since the cleavage of an inner bond creates a stable carbocation and a stable radical. Consequently, the mass signals of linear alkanes feature intense peaks in the middle of the mass-to-charge ratio plot with weaker peaks on either end. The fragmentation of each carbon-carbon bond with the release of a methyl group in each splitting leads to prominent peaks in the mass spectra...